公开(公告)号：US07252961B2

公开(公告)日：2007-08-07

申请号：US08895862

申请日：1997-07-17

申请人： Stephan Neuenhofer ,  Heinz-Jürgen Skrzipczyk ,  Peter Molz ,  Reinhard Käsmarker

发明人： Stephan Neuenhofer ,  Heinz-Jürgen Skrzipczyk ,  Peter Molz ,  Reinhard Käsmarker

IPC分类号： G01N33/533

CPC分类号： G01N33/58 ,  G01N33/54306 ,  G01N33/743 ,  G01N33/78 ,  Y10S436/824 ,  Y10S436/825

摘要： The invention relates to a method for the detection of an analyte in a competitive immunoassay in the presence of an analyte derivative and first and second receptor molecules, and to a kit for carrying out this detection method.

摘要翻译： 本发明涉及在分析物衍生物和第一和第二受体分子的存在下在竞争性免疫测定中检测分析物的方法以及用于实施该检测方法的试剂盒。

公开(公告)号：US06258551B1

公开(公告)日：2001-07-10

申请号：US09448824

申请日：1999-11-24

申请人： Stephan Neuenhofer ,  Reinhard Käsmarker

发明人： Stephan Neuenhofer ,  Reinhard Käsmarker

IPC分类号： G01N3353

CPC分类号： G01N33/582 ,  C12N9/96 ,  G01N33/532 ,  G01N33/58

摘要： The present invention relates to a method of carrying out an immunoassay in a multiphase system. A sample containing an analyte is brought into contact with a receptor A and a tracer. The analyte can either form a complex with the tracer, or counteract the formation of a complex of receptor A and tracer by competing with the tracer for binding to receptor A, or counteract the formation of a complex of receptor A and tracer by competing with receptor A. Receptor B is added and the signal is determined. In this method, receptor A and receptor B are suitably immobilized, ensuring that the tracer either cannot enter into any binding involving the simultaneous participation of receptors A and B or can enter into such a binding to only such a slight extent that it is nevertheless possible to detect and differentiate differing analyte concentrations.

摘要翻译： 本发明涉及在多相体系中进行免疫测定的方法。 将含有分析物的样品与受体A和示踪剂接触。 分析物可以与示踪物形成复合物，或通过与示踪剂竞争结合受体A来抵消受体A和示踪剂的复合物的形成，或通过与受体的竞争来抵消受体A和示踪物的复合物的形成 A.加入受体B，确定信号。 在该方法中，受体A和受体B被适当地固定，确保示踪剂不能进入涉及受体A和B的同时参与的任何结合，或者可以仅进行这样的结合，只有轻微的程度才可能 以检测和区分不同的分析物浓度。