公开(公告)号：US20120160995A1

公开(公告)日：2012-06-28

申请号：US13302793

申请日：2011-11-22

Applicant: Stephen J. GUNSTREAM ,  Mark Oldham

Inventor： Stephen J. GUNSTREAM ,  Mark Oldham

IPC: G01J1/58

CPC classification number: G01J1/58 ,  G01N21/278 ,  G01N21/6452 ,  Y10T436/10 ,  Y10T436/11 ,  Y10T436/112499 ,  Y10T436/113332

Abstract: Implementations of the present invention describe an apparatus for generating calibration factors for a spectral detector instrument. The calibration factors are derived from a calibration plate containing one or more spectral species in each well of the calibration plate. Each well is then exposed to an excitation source that causes the one or more spectral species in each of the wells to fluoresce. The signal response is measured and associated with each spectral species at each different well position in the calibration plate. Next, the measured signal response from each spectral species at each well position in the calibration plate is compared with a predetermined signal response for each spectral species. The results of this comparison can be used to determine a calibration factor for each well and spectral species to compensate for the difference between the measured signal response and the predetermined signal response.

Abstract translation: 本发明的实施方式描述了一种用于产生光谱检测器仪器的校准因子的装置。 校准因子来源于校准板的每个孔中含有一个或多个光谱种​​类的校准板。 然后将每个孔暴露于激发源，使得每个孔中的一个或多个光谱物质发荧光。 测量信号响应并与校准板中每个不同孔位置处的每个光谱种类相关联。 接下来，将来自校准板中每个孔位置处的每个光谱种类的测量信号响应与每个光谱种类的预定信号响应进行比较。 该比较的结果可用于确定每个孔和光谱种类的校准因子，以补偿测量的信号响应与预定信号响应之间的差异。

公开(公告)号：US20130041595A1

公开(公告)日：2013-02-14

申请号：US13584950

申请日：2012-08-14

Applicant: Stephen J. GUNSTREAM

Inventor： Stephen J. GUNSTREAM

IPC: G06F19/00

CPC classification number: C12Q1/6851 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/6486 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2201/12 ,  G01N2201/12746 ,  G16B30/00 ,  C12Q2545/113 ,  C12Q2545/101

Abstract: Systems and methods are provided for calibrating emission data or other information signals collected during a polymerase chain reaction (PCR), amplification reaction, assay, process, or other reaction. Calibration of multiple detectable materials can be achieved during a single cycle or run, or during a plurality of runs of the reaction. A reading from every well, container, or other support region of a sample support does not have to be taken. Interpolation can be used to determine values for emission data or other information signals that were not taken, or are unknown, using detected emission data, or other detected information signals. By calibrating the detected emission data and the interpolated data, a more accurate reading of emission data or information signal can be obtained.

Abstract translation: 提供了系统和方法，用于校准在聚合酶链式反应（PCR），扩增反应，测定，过程或其他反应期间收集的排放数据或其他信息信号。 可以在单个循环或运行期间或在多次反应运行期间实现多个可检测材料的校准。 来自样品支架的每个孔，容器或其他支撑区域的读数不必采取。 内插可用于确定未使用或未知的使用检测到的发射数据或其他检测到的信息信号的发射数据或其他信息信号的值。 通过校准检测到的发射数据和内插数据，可以获得更准确的发射数据或信息信号读数。

公开(公告)号：US20110231132A1

公开(公告)日：2011-09-22

申请号：US13070273

申请日：2011-03-23

Applicant: Stephen J. GUNSTREAM

Inventor： Stephen J. GUNSTREAM

IPC: G06F17/18 ,  G06F19/00

CPC classification number: C12Q1/6851 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/6486 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2201/12 ,  G01N2201/12746 ,  G06F19/22 ,  C12Q2545/113 ,  C12Q2545/101

Abstract: Systems and methods are provided for calibrating emission data or other information signals collected during a polymerase chain reaction (PCR), amplification reaction, assay, process, or other reaction. Calibration of multiple detectable materials can be achieved during a single cycle or run, or during a plurality of runs of the reaction. A reading from every well, container, or other support region of a sample support does not have to be taken. Interpolation can be used to determine values for emission data or other information signals that were not taken, or are unknown, using detected emission data, or other detected information signals. By calibrating the detected emission data and the interpolated data, a more accurate reading of emission data or information signal can be obtained.

Abstract translation: 提供了系统和方法，用于校准在聚合酶链式反应（PCR），扩增反应，测定，过程或其他反应期间收集的排放数据或其他信息信号。 可以在单个循环或运行期间或在多次反应运行期间实现多个可检测材料的校准。 来自样品支架的每个孔，容器或其他支撑区域的读数不必采取。 内插可用于确定未使用或未知的使用检测到的发射数据或其他检测到的信息信号的发射数据或其他信息信号的值。 通过校准检测到的发射数据和内插数据，可以获得更准确的发射数据或信息信号读数。