利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

787 条记录 (耗时 0.033 秒)

    Barcoded molecular standards
    2.
    发明授权
    Barcoded molecular standards 有权

    公开(公告)号：US12091705B2

    公开(公告)日：2024-09-17

    申请号：US17676523

    申请日：2022-02-21

    申请人： Thrive Earlier Detection Corp.

    发明人： Isaac A. Kinde ,  Howard B. Kaufman ,  Leonardo Hagmann

    IPC分类号： C12Q1/68 ,  C12Q1/6806

    CPC分类号： C12Q1/6806 ,  C12Q1/6806 ,  C12Q2535/122 ,  C12Q2545/101

    摘要： High throughput personal genomic testing has created a need for robust quality control mechanisms to track sample identity, reagent integrity, and other factors with significant influence on assay performance. A method of massively parallel sequencing using an accompanying barcoded molecular standard enables one to track nucleic acid analytes to identify them by project, lot, batch, or patient. The molecular standard contains sequences present in the analyte, allowing it to be processed simultaneously without any other additional reagents. Within the molecular standard, a calibrator sequence permits assessment of fidelity of sequence determination. Additional sequences in the molecular standard may be used to manipulate the molecular standard separate from the analyte. The molecular standard can be used to benchmark sequencing platforms and assess error rates.

    Method For Calibrating A Data Set Of A Target Analyte
    6.
    发明申请
    Method For Calibrating A Data Set Of A Target Analyte 审中-公开

    公开(公告)号：US20180336315A1

    公开(公告)日：2018-11-22

    申请号：US15777316

    申请日：2016-11-21

    申请人： SEEGENE, INC.

    发明人： Jong Yoon CHUN ,  Young Jo LEE ,  Han Bit LEE

    IPC分类号： G06F19/28

    CPC分类号： G06F19/28 ,  C12Q1/686 ,  G06F19/24 ,  C12Q2537/165 ,  C12Q2545/101

    摘要： The present invention relates to a method for calibrating a data set of a target analyte in a sample, wherein a normalization coefficient for calibrating the data set is provided by using a reference value, a reference cycle and the data set, and the calibrated data set is obtained by applying the normalization coefficient to the signal values of the data set. The present method is very effective in removing the inter- and intra-instrument signal variations of data sets. Furthermore, since the present method can be configured in software, the instant method is capable of being applied universally to various analytical instruments (e.g., a real-time PCR instrument) regardless of manufacturer. Accordingly, the method by the present invention would be very useful in diagnostic data analysis.

    CONSENSUS-BASED ALLELE DETECTION
    8.
    发明申请
    CONSENSUS-BASED ALLELE DETECTION 审中-公开

    公开(公告)号：US20180305756A1

    公开(公告)日：2018-10-25

    申请号：US15503338

    申请日：2015-08-12

    申请人： Progenika Biopharma S.A.

    发明人： Jorge Ochoa ,  David Arteta ,  Mária José Illescas ,  Monica Lopez ,  Marianne Stef ,  Diego Tejedor ,  Antonio Martínez

    IPC分类号： C12Q1/6881

    CPC分类号： C12Q1/6881 ,  C12Q1/6827 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2600/172 ,  C12Q2525/15 ,  C12Q2535/122 ,  C12Q2537/143 ,  C12Q2537/16 ,  C12Q2545/101

    摘要： The present invention provides a method for genotyping alleles in at least one homologous genetic loci set, comprising: (i) providing a DNA-containing sample that includes said at least one homologous genetic loci set; (ii) performing PCR amplification of regions of said homologous genetic loci set using consensus sequence-specific primers, wherein said consensus sequence-specific primers bind to consensus sequences that are common to a plurality of genes within the genetic loci set, thereby generating a pool of amplification products; (iii) sequencing a plurality of said amplification products in order to determine the relative proportion of each nucleotide at each position in a sequencing read; (iv) performing a sequence alignment between the sequencing read results of (iii) and at least one reference sequence, which reference sequence corresponds to one of the genes in said homologous genetic loci set; and (v) performing genotype calling of the allele or alleles in said sample based on the relative proportion of each nucleotide at each of a plurality of discriminant positions in said alignment. Also disclose are related products, kits and systems for performing the method.

    METHODS FOR QUANTITATIVE AMPLIFICATION AND DETECTION OVER A WIDE DYNAMIC RANGE
    10.
    发明申请
    METHODS FOR QUANTITATIVE AMPLIFICATION AND DETECTION OVER A WIDE DYNAMIC RANGE 审中-公开

    公开(公告)号：US20180216172A1

    公开(公告)日：2018-08-02

    申请号：US15843391

    申请日：2017-12-15

    申请人： GEN-PROBE INCORPORATED

    发明人： Daniel L. Kacian ,  Kenneth A. Browne

    IPC分类号： C12Q1/6851 ,  C12Q1/6813 ,  C12Q1/6865

    CPC分类号： C12Q1/6851 ,  C12Q1/6813 ,  C12Q1/6865 ,  C12Q2545/101 ,  C12Q2537/143

    摘要： Disclosed are compositions and methods for making differentiable amplicon species at unequal ratios using a single amplification system in a single vessel. The number of differentiable amplicons and their ratios to one another are chosen to span the required linear dynamic range for the amplification reaction and to accommodate limitations of the measuring system used to determine the amount of amplicon generated. Unequal amounts of distinguishable amplicon species are generated by providing unequal amounts of one or more amplification reaction components (e.g., distinguishable amplification oligomers, natural and unnatural NTP in an NTP mix, or the like). The amount of target nucleic acid present in a test sample is determined using the linear detection range generated from detection of one or more amplicon species having an amount within the dynamic range of detection.