公开(公告)号：US08198407B1

公开(公告)日：2012-06-12

申请号：US11207440

申请日：2005-08-19

申请人： Steven J. Burton ,  Baldev Baines ,  John Curling ,  Christopher Bryant ,  David John Hammond ,  Dwun-Hou Chen ,  Timothy Keith Hayes

发明人： Steven J. Burton ,  Baldev Baines ,  John Curling ,  Christopher Bryant ,  David John Hammond ,  Dwun-Hou Chen ,  Timothy Keith Hayes

IPC分类号： A61K38/00 ,  C12N5/07 ,  C12P21/04

CPC分类号： C07K14/745 ,  C07K1/22 ,  C07K14/775

摘要： The invention discloses methods for sequential protein isolation and purification from a biological sample by affinity chromatography. Affinity chromatography is conducted using ligands or ligand support complexes that selectively and specifically bind to proteins in the biological sample. The ligands or ligand support complexes were contacted sequentially in a predetermined order with the biological sample to allow each ligand or ligand-support complex to sequentially bind a protein from the biological sample.

摘要翻译： 本发明公开了通过亲和层析从生物样品中顺序分离和纯化蛋白质的方法。 使用选择性且特异性结合生物样品中的蛋白质的配体或配体载体复合物进行亲和层析。 配体或配体支持复合物以预定的顺序与生物样品顺序接触，以允许每个配体或配体支持复合物依次结合来自生物样品的蛋白质。