公开(公告)号：US20060046272A1

公开(公告)日：2006-03-02

申请号：US10928570

申请日：2004-08-27

申请人： Sue Chow ,  David Hedley ,  T. Shankey ,  Patricia Grom

发明人： Sue Chow ,  David Hedley ,  T. Shankey ,  Patricia Grom

IPC分类号： G01N33/567 ,  G01N33/53

CPC分类号： G01N33/80 ,  Y10T436/107497 ,  Y10T436/108331 ,  Y10T436/25 ,  Y10T436/25125 ,  Y10T436/2525 ,  Y10T436/25375

摘要： This invention is directed to a method for preparation of a biological sample for measurement of protein epitopes that allows for the preservation of intracellular protein epitopes and detection of signal transduction pathways based on the ability to capture transient activation states of the epitopes. The method provided by the invention allows for the rapid fixation of biological samples containing red blood cells, to ensure that epitopes of signal transduction molecules and other intracellular protein epitopes are preserved in the active state. The method of the invention further allows for lysis of red blood cells, thereby making it a useful method for cytometric analysis of biological samples, including, for example, whole blood, bone marrow aspirates, peritoneal fluids, and other red blood cell containing samples. The invention also provides a method to recover or “unmask” epitopes on intracellular antigens that have been made inaccessible by the cross linking fixative necessary to fix the sample. Significantly, the methods of the invention allow preservation and analysis of phospho-epitope levels in biological samples taken directly from patients to determine disease-specific characteristics.

摘要翻译： 本发明涉及一种用于制备用于测量蛋白质表位的生物样品的方法，其允许基于捕获表位的瞬时激活状态的能力来保留细胞内蛋白质表位和检测信号转导途径。 本发明提供的方法允许快速固定含有红细胞的生物样品，以确保信号转导分子和其他细胞内蛋白质表位的表位保持活跃状态​​。 本发明的方法还允许红细胞溶解，从而使其成为生物样品的细胞分析的有用方法，包括例如全血，骨髓抽吸物，腹膜液和其它含红细胞的样品。 本发明还提供了一种在细胞内抗原上恢复或“揭开”表位的方法，这些表位已经被固定样品所必需的交联固定剂制成。 显着地，本发明的方法允许保存和分析直接从患者获取的生物样品中的磷酸化表位水平，以确定疾病特异性特征。

公开(公告)号：US07803523B2

公开(公告)日：2010-09-28

申请号：US10928570

申请日：2004-08-27

申请人： Sue Chow ,  David Hedley ,  T. Vincent Shankey ,  Patricia Grom

发明人： Sue Chow ,  David Hedley ,  T. Vincent Shankey ,  Patricia Grom

IPC分类号： A01N1/02 ,  G01N1/00

CPC分类号： G01N33/80 ,  Y10T436/107497 ,  Y10T436/108331 ,  Y10T436/25 ,  Y10T436/25125 ,  Y10T436/2525 ,  Y10T436/25375

摘要： This invention is directed to a method for preparation of a biological sample for measurement of protein epitopes that allows for the preservation of intracellular protein epitopes and detection of signal transduction pathways based on the ability to capture transient activation states of the epitopes. The method provided by the invention allows for the rapid fixation of biological samples containing red blood cells, to ensure that epitopes of signal transduction molecules and other intracellular protein epitopes are preserved in the active state. The method of the invention further allows for lysis of red blood cells, thereby making it a useful method for cytometric analysis of biological samples, including, for example, whole blood, bone marrow aspirates, peritoneal fluids, and other red blood cell containing samples. The invention also provides a method to recover or “unmask” epitopes on intracellular antigens that have been made inaccessible by the cross linking fixative necessary to fix the sample. Significantly, the methods of the invention allow preservation and analysis of phospho-epitope levels in biological samples taken directly from patients to determine disease-specific characteristics.

摘要翻译： 本发明涉及一种用于制备用于测量蛋白质表位的生物样品的方法，其允许基于捕获表位的瞬时激活状态的能力来保留细胞内蛋白质表位和检测信号转导途径。 本发明提供的方法允许快速固定含有红细胞的生物样品，以确保信号转导分子和其他细胞内蛋白质表位的表位保持活跃状态​​。 本发明的方法还允许红细胞溶解，从而使其成为生物样品的细胞分析的有用方法，包括例如全血，骨髓抽吸物，腹膜液和其它含红细胞的样品。 本发明还提供了一种在细胞内抗原上恢复或“揭开”表位的方法，这些表位已经被固定样品所必需的交联固定剂制成。 显着地，本发明的方法允许保存和分析直接从患者获取的生物样品中的磷酸化表位水平，以确定疾病特异性特征。