公开(公告)号：US06183992B2

公开(公告)日：2001-02-06

申请号：US09230180

申请日：1999-03-10

申请人： Sun-Chang Kim ,  Jae Hyun Lee ,  Min Hyung Kang ,  Jeong Hyun Kim ,  Seung-Suh Hong ,  Hyun-Soo Lee

发明人： Sun-Chang Kim ,  Jae Hyun Lee ,  Min Hyung Kang ,  Jeong Hyun Kim ,  Seung-Suh Hong ,  Hyun-Soo Lee

IPC分类号： C12P2106

CPC分类号： C07K7/08 ,  C07K14/43563 ,  C07K14/43572 ,  C07K14/46 ,  C07K14/4723 ,  C07K14/815 ,  C07K2319/00 ,  C07K2319/23 ,  C12N15/62

摘要： The present invention provides a method for mass production of an antimicrobial peptide, which comprises the steps of: constructing a fusion gene containing a first gene encoding a negatively charged acidic peptide having at least two cysteine residues and a second gene encoding a positively charged basic antimicrobial peptide; transforming a host microorganism with an expression vector comprising the fusion gene; cultivating the transformed microorganism to express a fusion peptide containing the acidic peptide and antimicrobial peptide, and, recovering the expressed antimicrobial peptide. In accordance with the present invention, the inhibitory effect of the expressed antimicrobial peptide on the growth of the host microorganism can be dramatically minimized by fusing it with the acidic peptide. Accordingly, antimicrobial peptides can be produced massively from a recombinant microorganism regardless of the kind of the antimicrobial peptides.

摘要翻译： 本发明提供了一种大规模生产抗微生物肽的方法，其包括以下步骤：构建含有编码带有至少两个半胱氨酸残基的带负电荷的酸性肽的第一基因和编码带正电荷的碱性抗微生物剂的第二基因的融合基因 肽; 用包含融合基因的表达载体转化宿主微生物; 培养转化的微生物以表达含有酸性肽和抗微生物肽的融合肽，并回收表达的抗微生物肽。 根据本发明，表达的抗微生物肽对宿主微生物的生长的抑制作用可以通过与酸性肽融合来显着地最小化。 因此，抗微生物肽可以从重组微生物大量生产，而不管抗微生物肽的种类。

公开(公告)号：US06699689B1

公开(公告)日：2004-03-02

申请号：US09485147

申请日：2000-04-25

申请人： Jeong Hyun Kim ,  Min Hyung Kang ,  Jae Hyun Lee ,  Se Ho Park ,  Joo Won Lee ,  Seung Suh Hong ,  Hyun Soo Lee

发明人： Jeong Hyun Kim ,  Min Hyung Kang ,  Jae Hyun Lee ,  Se Ho Park ,  Joo Won Lee ,  Seung Suh Hong ,  Hyun Soo Lee

IPC分类号： C12P2106

CPC分类号： C12N15/72 ,  C07K14/4723 ,  C07K2319/00 ,  C12N9/1077 ,  C12N15/70

摘要： The present invention relates to DNA constructs that can produce antimicrobial materials efficiently from microorganisms and the preparation method thereof. The present invention also relates to the useful vector for the DNA construct. The DNA construct according to the present invention comprises a first gene coding for entire, a part of or a derivative of purF gene and a second gene coding for antimicrobial peptide. According to the present invention, antimicrobial peptides can be mass-produced by the following steps: preparing an expression vector containing a DNA construct comprising a first gene coding for an entire, a part of or a derivative of purF gene and a second gene coding for antimicrobial peptide; transforming the bacterial host cells with the above-mentioned vector, culturing the transformed cell to express the above-mentioned DNA construct; and recovering the above antimicrobial peptide.

摘要翻译： 本发明涉及能够从微生物有效生产抗微生物材料的DNA构建体及其制备方法。 本发明还涉及DNA构建体的有用载体。 根据本发明的DNA构建体包含编码purF基因的全部，一部分或衍生物的第一个基因和编码抗微生物肽的第二个基因。 根据本发明，通过以下步骤可以大量生产抗微生物肽：制备含有DNA构建体的表达载体，所述构建体包含编码purF基因全部或部分或衍生物的第一个基因，以及编码 抗菌肽; 用上述载体转化细菌宿主细胞，培养转化细胞以表达上述DNA构建体; 并回收上述抗微生物肽。