公开(公告)号：US20180252717A1

公开(公告)日：2018-09-06

申请号：US15760612

申请日：2016-09-14

Applicant: Swedish Health Services

Inventor： Parvinder HOTHI ,  Charles COBBS

IPC: G01N33/574

CPC classification number: G01N33/574 ,  A61K31/00 ,  A61K45/06 ,  C12N5/0695 ,  C12Q1/00 ,  G01N2800/7028

Abstract: A method of characterizing a glioblastoma multiforme (GBM) stem cell (GSC), comprising culturing the GSC to provide a culture, contacting a first set of aliquots of the culture with individual compounds selected from a panel of compounds, identifying two or more of the selected compounds that cause more than a threshold level of cell death in the first set of aliquots, and characterizing the GSC as suitable for treatment with one or more combinations comprising the two or more identified compounds. A panel of chemical compounds, the compounds selected by a method comprising surgically resecting the tumor, culturing a GSC derived from GBM tissue derived from a GBM tumor, contacting aliquots thereof with individual compounds selected from a panel of compounds, and identifying two or more of the selected compounds that cause more than a threshold level of cell death in the aliquots, thereby identifying the compounds.

公开(公告)号：US20210389299A1

公开(公告)日：2021-12-16

申请号：US17153198

申请日：2021-01-20

Applicant: Swedish Health Services

Inventor： Parvinder HOTHI ,  Charles COBBS

IPC: G01N33/50 ,  C12N5/09 ,  G01N33/574

Abstract: A method of characterizing a glioblastoma multiforme (GBM) stem cell (GSC), comprising culturing the GSC to provide a culture, contacting a first set of aliquots of the culture with individual compounds selected from a panel of compounds, identifying two or more of the selected compounds that cause more than a threshold level of cell death in the first set of aliquots, and characterizing the GSC as suitable for treatment with one or more combinations comprising the two or more identified compounds. A panel of anti-GBM chemotherapeutic compounds, the compounds selected by a method comprising surgically resecting the tumor, culturing a GSC derived from GBM tissue derived from a GBM tumor, contacting aliquots thereof with individual compounds selected from a panel of compounds, and identifying two or more of the selected compounds that cause more than a threshold level of cell death in the aliquots, thereby identifying the compounds.