公开(公告)号：US11714090B2

公开(公告)日：2023-08-01

申请号：US16771302

申请日：2018-12-13

Applicant: Syngenta Participations AG

Inventor： Michele Yarnell ,  Anne Chevrel ,  Olivier Kitten ,  Scott Young

IPC: C07K14/00 ,  G01N33/569 ,  G01N33/68

CPC classification number: G01N33/68 ,  C07K14/00 ,  G01N33/56961 ,  G01N2333/415

Abstract: The invention relates generally to synthetic non-antibody protein scaffolds (synNAPS) that differentially detect or quantitate a target insecticidal protein in a complex biological matrix comprising the target protein and a non-target insecticidal protein and to methods of using the synNAPS in immunoassays, and more particularly to monoclonal antibodies and immunoassays for the differential detection and quantitation of a wild-type crystal protein, such as a wild-type-Cry1Ab, from Bacillus thuringiensis and hybrid crystal proteins, which comprise all or a significant portion of the wild-type Cry protein in complex biological samples comprising both the wild-type Cry protein and one or more of the hybrid Cry proteins.

公开(公告)号：US20210190794A1

公开(公告)日：2021-06-24

申请号：US17271991

申请日：2019-08-14

Applicant: Syngenta Participations AG

Inventor： Scott Young ,  Richard Sessler ,  Gerson Graser ,  Rudolf Guilbaud ,  Michael Schirm ,  Maxim Isabelle

IPC: G01N33/68

Abstract: The invention relates generally to peptide biomarkers with specific ionization characteristics to directly quantify one or more transgenic target proteins in biological samples, including transgenic plant samples, by liquid chromatography coupled tandem mass spectrometry multiple reaction monitoring (MRM). The peptide biomarkers in combination with MRM-based methods may be used to quantify a single transgenic target protein or multiple transgenic target proteins within a stacked transgenic crop, such as maize, utilizing selected peptide biomarkers either alone or in combination. The present disclosure allows for broad based, reliable quantitation in different biological matrices, including plant matrices. The peptide biomarkers of the invention can further be used as trait biomarkers to support identification and/or selection of specific transgenic Events. Also provided are different peptide biomarker combinations that can be used to perform the methods of the invention.

公开(公告)号：US20210181204A1

公开(公告)日：2021-06-17

申请号：US16771302

申请日：2018-12-13

Applicant: Syngenta Participations AG

Inventor： Michele YARNELL ,  Anne CHEVREL ,  Olivier Kitten ,  Scott Young

IPC: G01N33/68 ,  G01N33/569 ,  C07K14/00

Abstract: The invention relates generally to synthetic non-antibody protein scaffolds (synNAPS) that differentially detect or quantitate a target insecticidal protein in a complex biological matrix comprising the target protein and a non-target insecticidal protein and to methods of using the synNAPS in immunoassays, and more particularly to monoclonal antibodies and immunoassays for the differential detection and quantitation of a wild-type crystal protein, such as a wild-type-Cry1Ab, from Bacillus thuringiensis and hybrid crystal proteins, which comprise all or a significant portion of the wild-type Cry protein in complex biological samples comprising both the wild-type Cry protein and one or more of the hybrid Cry proteins.