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公开(公告)号：US20210163971A1
公开(公告)日：2021-06-03
申请号：US17174515
申请日：2021-02-12
发明人： Timothy Kelliher , Satya Chintamanani , Brent Delzer , Michael L Nuccio , Robert Arthur Dietrich , Suresh Babu KADARU , Todd Lee Warner , William Paul Bullock
IPC分类号： C12N15/82 , C12Q1/6895 , C12N9/18 , A01H1/04 , A01H1/08
摘要： Provided are isolated cDNAs comprising a nucleotide sequence having at least 90% identity to SEQ ID NO: 33, SEQ ID NO: 37, SEQ ID NO: 52 or SEQ ID NO: 53. Also provided are expression cassettes; vectors; transgenic plant cells; plants, plant parts, and seeds; isolated polypeptides; amplicons and informative fragments of the presently disclosed nucleic acids; compositions that include amplification primer pairs; methods for producing plants that exhibit HI; methods for identifying the presence or absence of an allele associated with HI in a plant; methods for introgressing Haploid-inducing nucleotide sequences into plants; and methods for selecting parental plants predicted to produce progeny generations with plants that exhibit Haploid Induction trait.
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公开(公告)号：US10448588B2
公开(公告)日：2019-10-22
申请号：US15354912
申请日：2016-11-17
发明人： Satya Chintamanani , Timothy Kelliher , Brent Delzer , Michael Nuccio , Robert Arthur Dietrich , Suresh Babu Kadaru , Todd Warner , William Paul Bullock
摘要： Provided here are methods of using a mutated patatin-like phospholipase IIα (“pPLAIIα,” renamed here MATRILINEAL) to induce haploid induction in plants, cloning a pPLAIIα to induce haploid induction in plants, and genetically engineering a plant to contain a mutated pPLAIIα. Also provided are methods of applying topical and spray chemicals, lipids, and RNAi molecules to plants during pollination in order to induce haploid production. Further provided are methods of chemically treating plants during pollination to induce haploids while also reducing embryo abortion and increasing seed set.
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公开(公告)号：US12035666B2
公开(公告)日：2024-07-16
申请号：US17691549
申请日：2022-03-10
发明人： Timothy Kelliher , Satya Chintamanani , Brent Delzer , Michael L Nuccio , Robert Arthur Dietrich , Suresh Babu Kadaru , Todd Lee Warner , William Paul Bullock
IPC分类号： A01H1/08 , A01H1/04 , C12N9/18 , C12N15/82 , C12Q1/6895
CPC分类号： A01H1/08 , A01H1/045 , C12N9/18 , C12N15/8218 , C12N15/8247 , C12N15/8261 , C12N15/8287 , C12Q1/6895 , C12Y301/01002 , C12N2310/14 , C12N2310/20 , Y02A40/146
摘要： Provided here are methods of using a mutated patatin-like phospholipase IIα (“pPLAIIα,” renamed here MATRILINEAL) to induce haploid induction in plants, cloning a pPLAIIα to induce haploid induction in plants, and genetically engineering a plant to contain a mutated pPLAIIα. Also provided are methods of applying topical and spray chemicals, lipids, and RNAi molecules to plants during pollination in order to induce haploid production. Further provided are methods of chemically treating plants during pollination to induce haploids while also reducing embryo abortion and increasing seed set.
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公开(公告)号：US20240099213A1
公开(公告)日：2024-03-28
申请号：US18524717
申请日：2023-11-30
发明人： Timothy Kelliher , Satya Chintamanani , Brent Delzer , Michael L Nuccio , Robert Arthur Dietrich , Suresh Babu Kadaru , Todd Lee Warner , William Paul Bullock
IPC分类号： A01H1/08 , A01H1/04 , C12N9/18 , C12N15/82 , C12Q1/6895
CPC分类号： A01H1/08 , A01H1/045 , C12N9/18 , C12N15/8218 , C12N15/8247 , C12N15/8261 , C12N15/8287 , C12Q1/6895 , C12Y301/01002 , C12N2310/14 , C12N2310/20 , Y02A40/146
摘要： Provided here are methods of using a mutated patatin-like phospholipase IIα (“pPLAIIα,” renamed here MATRILINEAL) to induce haploid induction in plants, cloning a pPLAIIα to induce haploid induction in plants, and genetically engineering a plant to contain a mutated pPLAIIα. Also provided are methods of applying topical and spray chemicals, lipids, and RNAi molecules to plants during pollination in order to induce haploid production. Further provided are methods of chemically treating plants during pollination to induce haploids while also reducing embryo abortion and increasing seed set.
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公开(公告)号：US10954523B2
公开(公告)日：2021-03-23
申请号：US16218529
申请日：2018-12-13
发明人： Timothy Kelliher , Satya Chintamanani , Brent Delzer , Michael L Nuccio , Robert Arthur Dietrich , Suresh Babu Kadaru , Todd Lee Warner , William Paul Bullock
摘要： Provided are isolated cDNAs comprising a nucleotide sequence having at least 90% identity to SEQ ID NO: 33, SEQ ID NO: 37, SEQ ID NO: 52 or SEQ ID NO: 53. Also provided are expression cassettes; vectors; transgenic plant cells; plants, plant parts, and seeds; isolated polypeptides; amplicons and informative fragments of the presently disclosed nucleic acids; compositions that include amplification primer pairs; methods for producing plants that exhibit HI; methods for identifying the presence or absence of an allele associated with HI in a plant; methods for introgressing Haploid—inducing nucleotide sequences into plants; and methods for selecting parental plants predicted to produce progeny generations with plants that exhibit Haploid Induction trait.
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公开(公告)号：US10519456B2
公开(公告)日：2019-12-31
申请号：US16245923
申请日：2019-01-11
发明人： Qiudeng Que , Timothy Kelliher
IPC分类号： C12N15/82 , C12N15/10 , A01H1/08 , C12N15/113 , C12N15/65
摘要： The presently disclosed subject matter relates to using a haploid inducing line (whether existing or created) and transforming the haploid line so that it encodes cellular machinery capable of editing genes. The transformed haploid inducing line is used as a parent in a cross between two plants. During pollination, the parental gametes fuse to form an embryo; and the gene editing machinery is also delivered to the embryo at this time. During embryonic development, one set of parental chromosomes are lost, and the gene editing machinery operates on the remaining set of chromosomes. Thus, at least one haploid progeny with edited genes is produced from the cross.
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公开(公告)号：US10285348B2
公开(公告)日：2019-05-14
申请号：US15901464
申请日：2018-02-21
发明人： Timothy Kelliher , Qiudeng Que
IPC分类号： C12N15/82 , A01H1/08 , C07K14/415 , C12Q1/6895
摘要： The presently disclosed subject matter relates to using a haploid inducing line (whether existing or created) and transforming the haploid line so that it encodes cellular machinery capable of editing genes. The transformed haploid inducing line is used as a parent in a cross between two plants. During pollination, the parental gametes fuse to form an embryo; and the gene editing machinery is also delivered to the embryo at this time. During embryonic development, one set of parental chromosomes are lost, and the gene editing machinery operates on the remaining set of chromosomes. Thus, at least one haploid progeny with edited genes is produced from the cross.
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公开(公告)号：US20190136250A1
公开(公告)日：2019-05-09
申请号：US16245923
申请日：2019-01-11
发明人： Qiudeng Que , Timothy Kelliher
IPC分类号： C12N15/82 , C12N15/10 , C12N15/113 , C12N15/65
CPC分类号： C12N15/8213 , C12N15/102 , C12N15/113 , C12N15/65 , C12N15/8218 , C12N2310/20
摘要： The presently disclosed subject matter relates to using a haploid inducing line (whether existing or created) and transforming the haploid line so that it encodes cellular machinery capable of editing genes. The transformed haploid inducing line is used as a parent in a cross between two plants. During pollination, the parental gametes fuse to form an embryo; and the gene editing machinery is also delivered to the embryo at this time. During embryonic development, one set of parental chromosomes are lost, and the gene editing machinery operates on the remaining set of chromosomes. Thus, at least one haploid progeny with edited genes is produced from the cross.
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公开(公告)号：US20230060937A1
公开(公告)日：2023-03-02
申请号：US17327016
申请日：2019-12-17
发明人： Timothy Kelliher , Qiudeng Que
IPC分类号： C12N15/82 , C12Q1/6895
摘要： The presently disclosed subject matter relates to using a haploid inducing line (whether existing or created) and transforming the haploid line so that it encodes cellular machinery capable of editing genes. The transformed haploid inducing line is used as a parent in a cross between two plants. During pollination, the parental gametes fuse to form an embryo; and the gene editing machinery is also delivered to the embryo at this time. During embryonic development, one set of parental chromosomes are lost, and the gene editing machinery operates on the remaining set of chromosomes. Thus, at least one haploid progeny with edited genes is produced from the cross. The disclosure is also directed to methods of testing an edited haploid plant progeny for the presence of a first plant's genomic material.
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公开(公告)号：US20220192120A1
公开(公告)日：2022-06-23
申请号：US17691549
申请日：2022-03-10
发明人： Timothy Kelliher , Satya Chintamanani , Brent Delzer , Michael L Nuccio , Robert Arthur Dietrich , Suresh Babu KADARU , Todd Lee Warner , William Paul Bullock
IPC分类号： A01H1/08 , C12Q1/6895 , C12N15/82 , A01H1/04 , C12N9/18
摘要： Provided here are methods of using a mutated patatin-like phospholipase IIα (“pPLAIIα,” renamed here MATRILINEAL) to induce haploid induction in plants, cloning a pPLAIIα to induce haploid induction in plants, and genetically engineering a plant to contain a mutated pPLAIIα. Also provided are methods of applying topical and spray chemicals, lipids, and RNAi molecules to plants during pollination in order to induce haploid production. Further provided are methods of chemically treating plants during pollination to induce haploids while also reducing embryo abortion and increasing seed set.
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