公开(公告)号：US09783842B2

公开(公告)日：2017-10-10

申请号：US14372796

申请日：2013-01-18

Applicant: TECAN TRADING AG

Inventor： Joseph Mamone ,  Marc N. Feiglin ,  Howard Gamper

IPC: C12Q1/68

CPC classification number: C12Q1/6813 ,  C12Q1/6827 ,  C12Q2525/151 ,  C12Q2525/186

Abstract: In a method of deducing the number of repeat units in a selected short tandem repeat (STR) in a genomic sample, at least a single stranded target DNA generated from a genomic sample comprising a selected STR, an STR probe (P1,P1′), a reference probe (P2), and two blockers (B1,B2) are provided, and at least three differential hybridization experiments are carried out, based on which the number of STR probe oligonucleotides (P1,P1′) bound per target DNA strand in each differential hybridization experiment is determined. The method further comprises the step of comparing these numbers of STR probe oligonucleotides (P1,P1′) bound per target DNA strand in the differential hybridization experiments for deducing the number of repeat units in the selected STR on the single stranded target DNA strand. Also disclosed are kits for carrying out STR genotyping by differential hybridization.

公开(公告)号：US20150010908A1

公开(公告)日：2015-01-08

申请号：US14372796

申请日：2013-01-18

Applicant: TECAN TRADING AG

Inventor： Joseph Mamone ,  Marc N. Feiglin ,  Howard Gamper

IPC: C12Q1/68

CPC classification number: C12Q1/6813 ,  C12Q1/6827 ,  C12Q2525/151 ,  C12Q2525/186

Abstract: In a method of deducing the number of repeat units in a selected short tandem repeat (STR) in a genomic sample, at least a single stranded target DNA generated from a genomic sample comprising a selected STR, an STR probe (P1,P1′), a reference probe (P2), and two blockers (B1,B2) are provided, and at least three differential hybridization experiments are carried out, based on which the number of STR probe oligonucleotides (P1,P1′) bound per target DNA strand in each differential hybridization experiment is determined. The method further comprises the step of comparing these numbers of STR probe oligonucleotides (P1,P1′) bound per target DNA strand in the differential hybridization experiments for deducing the number of repeat units in the selected STR on the single stranded target DNA strand. Also disclosed are kits for carrying out STR genotyping by differential hybridization.

Abstract translation: 在基因组样品中推定所选短串联重复序列（STR）中的重复单元数的方法中，至少由包含选定的STR，STR探针（P1，P1'）的基因组样品产生的单链靶DNA， ，提供参考探针（P2）和两个阻断剂（B1，B2），并进行至少三个差异杂交实验，基于此，每个靶DNA链结合的STR探针寡核苷酸（P1，P1'）的数量 在每个差异杂交实验中确定。 该方法还包括在差异杂交实验中比较在每个靶DNA链上结合的STR探针寡核苷酸（P1，P1'）数目，以推断单链靶DNA DNA链上所选STR中重复单元数目的步骤。 还公开了用于通过差异杂交进行STR基因分型的试剂盒。