公开(公告)号：US10989661B2

公开(公告)日：2021-04-27

申请号：US15570636

申请日：2016-04-26

Applicant: THE BOARD OF REGENTS OF THE UNIVERSITY OF TEXAS SYSTEM

Inventor： Reto P. Fiolka ,  Kevin M. Dean

IPC: G01N21/64 ,  G02B21/00 ,  G02B21/36 ,  G02B21/16

Abstract: Light-sheet fluorescence microscopy (LSFM) affords highly parallelized 3D imaging with optical sectioning capability and minimal light exposure. However, using Gaussian beams for light-sheet generation results in a trade-off between beam waist thickness and the area over which the beam can approximate a light-sheet. Novel techniques for LSFM are disclosed that uses extended focusing and/or laser line focuses to produce divergence free light-sheets with near diffraction-limited resolution and uniform intensity distribution.