公开(公告)号：US20220142936A1

公开(公告)日：2022-05-12

申请号：US17521414

申请日：2021-11-08

Applicant: THE REGENTS OF THE UNIVERSITY OF MICHIGAN

Inventor： Joerg LAHANN ,  Nahal HABIBI ,  Jason V. GREGORY ,  Maria CASTRO ,  Pedro LOWENSTEIN ,  Ava MAUSER ,  Padma KADIYALA ,  Daniel F. QUEVEDO ,  Felipe NUNEZ

IPC: A61K9/51 ,  A61P35/00 ,  A61K31/7105

Abstract: Protein-based nanoparticles for treating cancer, such as those characterized by intracranial tumors, and methods of forming such protein-based nanoparticles via electrohydrodynamic jetting methods are provided. The nanoparticle may comprise a water-soluble protein having an average molecular weight of ≥about 8 kDa and ≤about 700 kDa. In certain variations, the water-soluble protein is cross-linked (e.g., with an optional crosslinking agent) and defines a mesh structure having an average linear mesh size of ≥about 1 nm to ≤about 4 nm. The nanoparticle may have a transcription factor such as a therapeutic nucleic acid in the mesh structure. Methods of making such nanoparticles may include jetting a liquid comprising the water-soluble protein through a nozzle, followed by exposing the liquid to an electric field sufficient to solidify the liquid and form the protein-based nanoparticles described above.