THERAPEUTIC PROTEIN-BASED NANOPARTICLES FOR TREATING CANCER

    公开(公告)号:US20220142936A1

    公开(公告)日:2022-05-12

    申请号:US17521414

    申请日:2021-11-08

    Abstract: Protein-based nanoparticles for treating cancer, such as those characterized by intracranial tumors, and methods of forming such protein-based nanoparticles via electrohydrodynamic jetting methods are provided. The nanoparticle may comprise a water-soluble protein having an average molecular weight of ≥about 8 kDa and ≤about 700 kDa. In certain variations, the water-soluble protein is cross-linked (e.g., with an optional crosslinking agent) and defines a mesh structure having an average linear mesh size of ≥about 1 nm to ≤about 4 nm. The nanoparticle may have a transcription factor such as a therapeutic nucleic acid in the mesh structure. Methods of making such nanoparticles may include jetting a liquid comprising the water-soluble protein through a nozzle, followed by exposing the liquid to an electric field sufficient to solidify the liquid and form the protein-based nanoparticles described above.

    HIERARCHICALLY STRUCTURED PROTEIN MATERIALS FOR THREE DIMENSIONAL (3D) CELLULAR SUPPORT SYSTEMS

    公开(公告)号:US20190144818A1

    公开(公告)日:2019-05-16

    申请号:US16300499

    申请日:2017-05-11

    Abstract: A cellular support system comprises a three-dimensional scaffold structure comprising at least one void. At least one suspended protein bridge spans across the at least one void in the three-dimensional scaffold structure. The suspended protein bridge is capable of supporting cells and promotes three-dimensional cellular growth. In certain aspects, the protein in the suspended protein bridge is an extracellular matrix protein, such as collagens, laminins, fibronectins, and combinations thereof. Such a cellular support system supports thriving cell cultures in three-dimensions emulating cell growth in vivo in an extracellular matrix, including promoting cell remodeling. Methods for making such cellular support systems are also provided.

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