公开(公告)号：US20210345601A1

公开(公告)日：2021-11-11

申请号：US17269238

申请日：2019-08-16

Applicant: THE UNIVERSITY OF BRITISH COLUMBIA ,  UNIVERSITY HEALTH NETWORK

Inventor： Marcelo CYPEL ,  Aizhou WANG ,  Shafique KESHAVJEE ,  Stephen G. WITHERS ,  Peter RAHFELD ,  Jayachandran KIZHAKKEDATHU

IPC: A01N1/02 ,  C12N9/80 ,  C12N9/24

Abstract: Provided herein are perfusion fluids for enzymatically cleaving A-antigens from a donor organ, and methods, uses, associated therewith. In particular, the perfusion fluids comprise two enzymes, GalNAcDeacetylase and Galactosaminidase and the fluids may further comprise a buffered extracellular solution and/or a crowing agent. Furthermore, the compositions described herein were found to have activity at temperatures and pH levels suitable for cell viability.

公开(公告)号：US20240294895A1

公开(公告)日：2024-09-05

申请号：US18441770

申请日：2024-02-14

Applicant: THE UNIVERSITY OF BRITISH COLUMBIA ,  UNIVERSITY HEALTH NETWORK

Inventor： Marcelo CYPEL ,  Aizhou WANG ,  Shafique KESHAVJEE ,  Stephen G. WITHERS ,  Peter RAHFELD ,  Jayachandran KIZHAKKEDATHU

IPC: C12N9/80 ,  A01N1/02 ,  C12N9/24 ,  C12N11/00

CPC classification number: C12N9/80 ,  A01N1/0226 ,  C12N9/2402 ,  C12N11/00 ,  C12Y302/01049 ,  C12Y305/01025 ,  C07K2319/00

Abstract: Provided herein are perfusion fluids for enzymatically cleaving A-antigens from a donor organ, and methods. uses. associated therewith. In particular, the perfusion fluids comprise two enzymes. GalNAcDeacetylase and Galactosaminidase and the fluids may further comprise a buffered extracellular solution and/or a crowing agent. Furthermore. the compositions described herein were found to have activity at temperatures and ph levels suitable for cell viability.

公开(公告)号：US20250011749A1

公开(公告)日：2025-01-09

申请号：US18628538

申请日：2024-04-05

Applicant: The University of British Columbia

Inventor： Stephen G. WITHERS ,  Peter RAHFELD ,  Jayachandran KIZHAKKEDATHU

IPC: C12N9/80 ,  A01N1/02 ,  C12N9/24 ,  C12N11/00

Abstract: Provided herein are enzymatic compositions for carbohydrate antigen cleavage, methods, uses, apparatuses and sys-O tems associated therewith. In particular, the composition comprises two enzymes, GalNAcDeacetylase and Galactosaminidase and the el composition may further comprise a crowding agent. Furthermore, the compositions described herein were found to have activity a C) temperatures and pH levels suitable for cell viability.

公开(公告)号：US20210324361A1

公开(公告)日：2021-10-21

申请号：US17269235

申请日：2019-08-16

Applicant: THE UNIVERSITY OF BRITISH COLUMBIA

Inventor： Stephen G. WITHERS ,  Peter RAHFELD ,  Jayachandran KIZHAKKEDATHU

IPC: C12N9/80 ,  C12N9/24 ,  A01N1/02 ,  C12N11/00

Abstract: Provided herein are enzymatic compositions for carbohydrate antigen cleavage, methods, uses, apparatuses and systems associated therewith. In particular, the composition comprises two enzymes, GalNAcDeacetylase and Galactosaminidase and the composition may further comprise a crowding agent. Furthermore, the compositions described herein were found to have activity a temperatures and pH levels suitable for cell viability.