公开(公告)号：US20200032330A1

公开(公告)日：2020-01-30

申请号：US16491278

申请日：2018-03-08

Applicant: The University of Chicago

Inventor： Chuan HE ,  Boxuan Simen ZHAO ,  Pradnya NARKHEDE ,  Chang LIU ,  Xiaolong CUI

IPC: C12Q1/6844 ,  C12Q1/6806

Abstract: Methods, compositions and kits are provided to amplify the amount of genomic methylated DNA can by subsequently analyzed and/or sequenced. It has particular use with small amounts of DNA, including, but not limited to cell free DNA samples. In some embodiments, the ratio of polymerase and methyltransferase is controlled in order to provide maximum yields. In some embodiments, a dual primase/polymerase is used.

公开(公告)号：US20250092449A1

公开(公告)日：2025-03-20

申请号：US18954222

申请日：2024-11-20

Applicant: THE UNIVERSITY OF CHICAGO

Inventor： Chuan HE ,  Boxuan Simen ZHAO ,  Pradnya NARKHEDE ,  Chang LIU ,  Xiaolong CUI

IPC: C12Q1/6844 ,  C12Q1/6806

Abstract: Methods, compositions and kits are provided to amplify an amount of genomic methylated DNA that can be subsequently analyzed and/or sequenced. It has particular use with small amounts of DNA, including, but not limited to cell free DNA samples. In some embodiments, the ratio of polymerase and methyltransferase is controlled in order to provide maximum yields. In some embodiments, a dual primase/polymerase is used.

公开(公告)号：US20220033890A1

公开(公告)日：2022-02-03

申请号：US17448958

申请日：2021-09-27

Applicant: The University of Chicago

Inventor： Chuan HE ,  Boxuan Simen ZHAO ,  Pradnya NARKHEDE ,  Chang LIU ,  Xiaolong CUI

IPC: C12Q1/6844 ,  C12Q1/6806

Abstract: Methods, compositions and kits are provided to amplify the amount of genomic methylated DNA can by subsequently analyzed and/or sequenced. It has particular use with small amounts of DNA, including, but not limited to cell free DNA samples. In some embodiments, the ratio of polymerase and methyltransferase is controlled in order to provide maximum yields. In some embodiments, a dual primase/polymerase is used.