公开(公告)号：US10261090B2

公开(公告)日：2019-04-16

申请号：US15116661

申请日：2015-02-24

申请人： THE UNIVERSITY OF TOKYO

发明人： Yasuteru Urano ,  Mako Kamiya ,  Keitaro Umezawa ,  Masafumi Yoshida

IPC分类号： G01N33/68 ,  C09B11/08 ,  C09B11/24 ,  C09B11/28 ,  C09B19/00 ,  C09K11/07 ,  G01N21/64 ,  C07F7/08 ,  G01N33/58 ,  C09K11/06

摘要： [Problem] To provide the following: a novel fluorescent probe for detecting a compound, such as glutathione, that contains a —SH group; a detection method using said fluorescent probe; and a detection kit containing said probe.[Solution] A fluorescent probe for detecting a compound containing an —SH group, wherein the fluorescent probe comprises a compound represented by formula (I) or a salt thereof (In the formula, X represents Si(Ra)(Rb), Ge(Ra)(Rb), Sn(Ra)(Rb), C(Ra)(Rb), or O (wherein Ra and Rb each independently represent a hydrogen atom or alkyl group); R1 represents a hydrogen atom, or 1-4 identical or different substituents independently selected from the group consisting of a cyano group, alkyl group, carboxyl group, ester group, alkoxy group, amide group, and azide group, each of which may be optionally substituted; R2 represents a hydrogen atom, halogen atom, hydroxyl group, cyano group, or an alkyl group, alkynyl group, alkoxy group, aryl, or heteroaryl, each of which may be optionally substituted; R3 and R4 each independently represent a hydrogen atom or 1-3 identical or different substituents independently selected from the group consisting of a hydroxyl group, halogen atom, or an alkyl group, sulfo group, carboxyl group, ester group, amide group and azide group, each of which may be optionally substituted; R5, R6, R7 and R8 each independently represent a hydrogen atom or alkyl group, wherein, R5 or R6, respectively together with R3, may form a ring structure including the nitrogen atoms bonded thereto, or R7 or R8, respectively together with R4, may form a ring structure including the nitrogen atoms bonded thereto).