公开(公告)号：US20230076614A1

公开(公告)日：2023-03-09

申请号：US17613911

申请日：2020-06-05

Applicant: THE UNIVERSITY OF WARWICK

Inventor： Nicholas R. WATERFIELD ,  Joseph HEALEY ,  Alexia HAPESHI

IPC: C07K14/195 ,  B82Y5/00

Abstract: The present invention provides use of a Photorhabdus Virulence Cassettes (PVC) effector leader sequence, for packaging a payload into a PVC Needle Complex, and related methods for manufacturing a packaged PVC Needle Complex. The payload is one or more selected from a polypeptide, a nucleic acid, or a combination thereof, and the leader sequence and the payload form an effector fusion that is distinct from a wild-type PVC effector protein.