公开(公告)号：US20220403343A1

公开(公告)日：2022-12-22

申请号：US17779275

申请日：2019-11-26

Applicant: THE UNIVERSITY OF WESTERN AUSTRALIA ,  MONASH UNIVERSITY

Inventor： Ryan LISTER ,  Sam BUCKBERRY ,  Jose POLO ,  Xiaodong LIU

IPC: C12N5/074

Abstract: The present invention relates to methods for producing an induced pluripotent stem (iPSC), the method comprising the following steps in order: culturing a somatic cell in a first culture condition adapted to promote the reprogramming of the cell towards a pluripotent state; culturing the cell in a second culture condition adapted to promote a hypomethylated DNA state in the cell; and culturing the cell in a third culture condition adapted to promote a primed pluripotent state, thereby producing an iPSC from a somatic cell. The invention also relates to cells and compositions resulting from those methods.