公开(公告)号：US20160137994A1

公开(公告)日：2016-05-19

申请号：US14436833

申请日：2013-10-17

Applicant: TOKUSHIMA UNIVERSITY ,  MEIJI PHARMACEUTICAL UNIVERSITY

Inventor： Koji ITO ,  Hitoshi SAKURABA ,  Daisuke TSUJI

IPC: C12N9/24 ,  A61K38/47

CPC classification number: C12N9/2402 ,  A61K38/00 ,  A61K38/47 ,  C12Y302/01052

Abstract: Provided is a modified β-subunit of human β-hexosaminidase which has the activity derived from the α-subunit of wild-type human β-hexosaminidase and has the resistance to protease. A protein comprising an amino acid sequence having substitutions of the 312th to the 318th amino acids with glycine, serine, glutamic acid, proline, serine, glycine and threonine in order, respectively, in an amino acid sequence of a β-subunit of wild-type human β-hexosaminidase.

Abstract translation: 提供了具有衍生自野生型人类 - 半胱胺糖苷酶的α-亚单位的活性的人类 - 半胱胺酶的修饰和亚基，并具有对蛋白酶的抗性。 包含具有312至318位氨基酸的氨基酸序列的氨基酸序列的氨基酸序列分别与甘氨酸，丝氨酸，谷氨酸，脯氨酸，丝氨酸，甘氨酸和苏氨酸的氨基酸序列分别在野生型 型人类和半乳糖胺酶。