公开(公告)号：US20140163388A1

公开(公告)日：2014-06-12

申请号：US14099126

申请日：2013-12-06

Applicant: TOKYO UNIVERSITY OF SCIENCE FOUNDATION ,  SHIMADZU CORPORATION

Inventor： Tomoki SASAYAMA ,  Satoshi YAMAMOTO ,  Kohei SOGA

IPC: A61B5/00

CPC classification number: A61B5/0071 ,  F04C2270/041 ,  G01N21/6456

Abstract: Measuring light emitted from a laser light source and split into four light rays through optical fibers is emitted into a space from emission units, and bent upward at a mirror. The measuring light passes through a window in the center of the sample stage and strikes a lower surface of the biological sample placed on the sample stage. A portion of fluorescence emitted through excitation by the measuring light passes through the window, is bent in an opposite direction from the measuring light by the mirror, and guided to a fluorescence camera. An objective lens, and a spectroscopic unit for separating visible wavelength components are horizontally arranged between the camera and the mirror. Although the projection of the measuring light and the detection of the fluorescence are performed perpendicularly to the biological sample, the optical components and elements are horizontally arranged.

Abstract translation: 测量从激光光源发射并通过光纤分解成四个光线的光从发射单元发射到空间中，并以镜子向上弯曲。 测量光通过样品台中心的窗口，并撞击放置在样品台上的生物样品的下表面。 通过测量光的激发发出的一部分荧光通过窗口，通过反射镜沿与测量光相反的方向弯曲，并被引导到荧光相机。 物镜和用于分离可见波长分量的分光单元水平地布置在相机和反射镜之间。 虽然测量光的投影和荧光的检测是垂直于生物样品进行的，但光学部件和元件水平排列。