公开(公告)号：US20180355351A1

公开(公告)日：2018-12-13

申请号：US16006581

申请日：2018-06-12

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： Rebecca NUGENT ,  Siyuan CHEN ,  Elian LEE ,  Nathan RAYNARD

IPC: C12N15/10 ,  C12N9/00 ,  C12N9/12 ,  C12P19/34 ,  C12Q1/6855 ,  C12Q1/6869

Abstract: Provided herein are methods, systems, and compositions for seamless nucleic acid assembly. Methods, systems, and compositions as provided herein provide for efficient assembly of nucleic acids without primer removal. Methods, systems, and compositions for seamless nucleic acid assembly comprise use of an endonuclease or exonuclease, optionally in conjunction with additional enzymes to assemble nucleic acids or polynucleotides.

公开(公告)号：US20220325278A1

公开(公告)日：2022-10-13

申请号：US17720210

申请日：2022-04-13

Applicant: Twist Bioscience Corporation

Inventor： Rebecca NUGENT ,  Siyuan CHEN ,  Elian LEE ,  Nathan RAYNARD

IPC: C12N15/10 ,  C12N9/00 ,  C12N9/12 ,  C12P19/34 ,  C12Q1/6869 ,  C12Q1/6855 ,  C12Q1/6811

Abstract: Provided herein are methods, systems, and compositions for seamless nucleic acid assembly. Methods, systems, and compositions as provided herein provide for efficient assembly of nucleic acids without primer removal. Methods, systems, and compositions for seamless nucleic acid assembly comprise use of an endonuclease or exonuclease, optionally in conjunction with additional enzymes to assemble nucleic acids or polynucleotides.

公开(公告)号：US20200283760A1

公开(公告)日：2020-09-10

申请号：US16879705

申请日：2020-05-20

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： Rebecca NUGENT ,  Siyuan CHEN ,  Elian LEE ,  Nathan RAYNARD

IPC: C12N15/10 ,  C12N9/00 ,  C12N9/12 ,  C12P19/34 ,  C12Q1/6869 ,  C12Q1/6855 ,  C12Q1/6811

Abstract: Provided herein are methods, systems, and compositions for seamless nucleic acid assembly. Methods, systems, and compositions as provided herein provide for efficient assembly of nucleic acids without primer removal. Methods, systems, and compositions for seamless nucleic acid assembly comprise use of an endonuclease or exonuclease, optionally in conjunction with additional enzymes to assemble nucleic acids or polynucleotides.

公开(公告)号：US20230115861A1

公开(公告)日：2023-04-13

申请号：US17820856

申请日：2022-08-18

Applicant: Twist Bioscience Corporation

Inventor： Rebecca NUGENT ,  Siyuan CHEN ,  Nathan RAYNARD

IPC: C12Q1/6806 ,  C12N15/10 ,  C12P19/34

Abstract: Provided herein are methods, systems, and compositions for assembly of covalently closed double stranded nucleic acids. Provided herein are methods, systems, and compositions for assembly covalently closed double stranded nucleic acids for use in various downstream processes.

公开(公告)号：US20220243195A1

公开(公告)日：2022-08-04

申请号：US17721680

申请日：2022-04-15

Applicant: Twist Bioscience Corporation

Inventor： Rebecca NUGENT ,  Siyuan CHEN ,  Ross KETTLEBOROUGH ,  Elian LEE ,  Nathan RAYNARD

IPC: C12N15/10

Abstract: Provided herein are methods, systems, and compositions for efficient nucleic acid assembly. Nucleic acid assembly may comprise assembly of variants comprising paired homology.

公开(公告)号：US20210071168A1

公开(公告)日：2021-03-11

申请号：US16906555

申请日：2020-06-19

Applicant: Twist Bioscience Corporation

Inventor： Rebecca NUGENT ,  Siyuan CHEN ,  Ross KETTLEBOROUGH ,  Elian LEE ,  Nathan RAYNARD

IPC: C12N15/10

Abstract: Provided herein are methods, systems, and compositions for efficient nucleic acid assembly. Nucleic acid assembly may comprise assembly of variants comprising paired homology.