公开(公告)号：US06238920B1

公开(公告)日：2001-05-29

申请号：US08018546

申请日：1993-02-17

申请人： Takashi Nagai ,  Akira Okano ,  Masashi Takahashi ,  Takayoshi Kariya ,  Hiroya Kadokawa ,  Hitomi Hirako ,  Masashige Kuwayama ,  Seizo Hamano

发明人： Takashi Nagai ,  Akira Okano ,  Masashi Takahashi ,  Takayoshi Kariya ,  Hiroya Kadokawa ,  Hitomi Hirako ,  Masashige Kuwayama ,  Seizo Hamano

IPC分类号： C12N502

CPC分类号： A01N1/02 ,  A01N1/0226

摘要： A method of culturing bovine embryos by culturing said bovine embryos in a medium containing thiol compound, and also a method of transporting bovine embryos by transporting said bovine embryos in a medium containing thiol compound are disclosed. According to the present invention, it is possible to culture bovine embryos up to blastocyst stage efficiently in the medium containing low molecular thiol compound without in vivo culture or coculture using feeder cells. Also when low molecular thiol compound is added to the transportation medium for bovine embryos, almost 100% embryos are still viable after long distant transportation.

摘要翻译： 公开了一种通过在含有硫醇化合物的培养基中培养所述牛胚胎培养牛胚胎的方法，以及通过在含有硫醇化合物的培养基中转运所述牛胚胎来运输牛胚胎的方法。根据本发明， 在含有低分子硫醇化合物的培养基中有效培养牛胚胎至胚泡期，而不使用饲养细胞进行体内培养或共培养。 此外，当将低分子硫醇化合物加入到牛胚胎的运输介质中时，几乎100％的胚胎在远距离运输后仍然是可行的。