公开(公告)号：US20110136142A1

公开(公告)日：2011-06-09

申请号：US12961140

申请日：2010-12-06

申请人： Takayoshi OYAMADA ,  Junichi Katada ,  Kenji Narahara ,  Daisuke Ogata ,  Tomonori Takeshige ,  Akihiko Onodera

发明人： Takayoshi OYAMADA ,  Junichi Katada ,  Kenji Narahara ,  Daisuke Ogata ,  Tomonori Takeshige ,  Akihiko Onodera

IPC分类号： G01N33/53

CPC分类号： G01N33/54393 ,  G01N33/558 ,  G01N33/585

摘要： It is an object of the present invention to provide an immunochromatography method capable of measuring a sample in a low concentration range and a high concentration range by setting the measurable range of the sample much wider than the conventional measurable range. The present invention provides an immunochromatography method, which comprises: spreading a test substance, a first labeling substance modified with a first binding substance that binds to the test substance, and a second labeling substance modified with the first binding substance that binds to the test substance, which are in a mixed state, on an insoluble carrier; and capturing the test substance, the first labeling substance, and/or the second labeling substance at a reaction site on the insoluble carrier having a substance having ability to bind to the first binding substance that binds to the test substance, so as to detect the test substance, wherein the first labeling substance is a labeling substance that causes an amplification reaction, and the second labeling substance is a labeling substance that does not substantially influence on the amplification reaction.

摘要翻译： 本发明的目的是提供一种免疫色谱法，其能够通过将样品的可测量范围设定为比现有的可测量范围宽得多的范围来测量低浓度范围和高浓度范围的样品。 本发明提供了一种免疫色谱法，其包括：扩散测试物质，用与测试物质结合的第一结合物质改性的第一标记物质和与第一结合物质相结合的第二标记物质， 在不溶性载体上处于混合状态; 以及在具有结合到与测试物质结合的第一结合物质的能力的物质的不溶性载体上的反应部位捕获测试物质，第一标记物质和/或第二标记物质，以便检测 测试物质，其中所述第一标记物质是引起扩增反应的标记物质，所述第二标记物质是对扩增反应没有实质影响的标记物质。

公开(公告)号：US08999730B2

公开(公告)日：2015-04-07

申请号：US12961140

申请日：2010-12-06

申请人： Takayoshi Oyamada ,  Junichi Katada ,  Kenji Narahara ,  Daisuke Ogata ,  Tomonori Takeshige ,  Akihiko Onodera

发明人： Takayoshi Oyamada ,  Junichi Katada ,  Kenji Narahara ,  Daisuke Ogata ,  Tomonori Takeshige ,  Akihiko Onodera

IPC分类号： G01N33/58 ,  G01N33/543 ,  G01N33/558

CPC分类号： G01N33/54393 ,  G01N33/558 ,  G01N33/585

摘要： It is an object of the present invention to provide an immunochromatography method capable of measuring a sample in a low concentration range and a high concentration range by setting the measurable range of the sample much wider than the conventional measurable range. The present invention provides an immunochromatography method, which comprises: spreading a test substance, a first labeling substance modified with a first binding substance that binds to the test substance, and a second labeling substance modified with the first binding substance that binds to the test substance, which are in a mixed state, on an insoluble carrier; and capturing the test substance, the first labeling substance, and/or the second labeling substance at a reaction site on the insoluble carrier having a substance having ability to bind to the first binding substance that binds to the test substance, so as to detect the test substance, wherein the first labeling substance is a labeling substance that causes an amplification reaction, and the second labeling substance is a labeling substance that does not substantially influence on the amplification reaction.

摘要翻译： 本发明的目的是提供一种免疫色谱法，其能够通过将样品的可测量范围设定为比现有的可测量范围宽得多的范围来测量低浓度范围和高浓度范围的样品。 本发明提供了一种免疫色谱法，其包括：扩散测试物质，用与测试物质结合的第一结合物质改性的第一标记物质和与第一结合物质相结合的第二标记物质， 在不溶性载体上处于混合状态; 以及在具有结合到与测试物质结合的第一结合物质的能力的物质的不溶性载体上的反应部位捕获测试物质，第一标记物质和/或第二标记物质，以便检测 测试物质，其中所述第一标记物质是引起扩增反应的标记物质，所述第二标记物质是对扩增反应没有实质影响的标记物质。