公开(公告)号：US08999730B2

公开(公告)日：2015-04-07

申请号：US12961140

申请日：2010-12-06

申请人： Takayoshi Oyamada ,  Junichi Katada ,  Kenji Narahara ,  Daisuke Ogata ,  Tomonori Takeshige ,  Akihiko Onodera

发明人： Takayoshi Oyamada ,  Junichi Katada ,  Kenji Narahara ,  Daisuke Ogata ,  Tomonori Takeshige ,  Akihiko Onodera

IPC分类号： G01N33/58 ,  G01N33/543 ,  G01N33/558

CPC分类号： G01N33/54393 ,  G01N33/558 ,  G01N33/585

摘要： It is an object of the present invention to provide an immunochromatography method capable of measuring a sample in a low concentration range and a high concentration range by setting the measurable range of the sample much wider than the conventional measurable range. The present invention provides an immunochromatography method, which comprises: spreading a test substance, a first labeling substance modified with a first binding substance that binds to the test substance, and a second labeling substance modified with the first binding substance that binds to the test substance, which are in a mixed state, on an insoluble carrier; and capturing the test substance, the first labeling substance, and/or the second labeling substance at a reaction site on the insoluble carrier having a substance having ability to bind to the first binding substance that binds to the test substance, so as to detect the test substance, wherein the first labeling substance is a labeling substance that causes an amplification reaction, and the second labeling substance is a labeling substance that does not substantially influence on the amplification reaction.

摘要翻译： 本发明的目的是提供一种免疫色谱法，其能够通过将样品的可测量范围设定为比现有的可测量范围宽得多的范围来测量低浓度范围和高浓度范围的样品。 本发明提供了一种免疫色谱法，其包括：扩散测试物质，用与测试物质结合的第一结合物质改性的第一标记物质和与第一结合物质相结合的第二标记物质， 在不溶性载体上处于混合状态; 以及在具有结合到与测试物质结合的第一结合物质的能力的物质的不溶性载体上的反应部位捕获测试物质，第一标记物质和/或第二标记物质，以便检测 测试物质，其中所述第一标记物质是引起扩增反应的标记物质，所述第二标记物质是对扩增反应没有实质影响的标记物质。

公开(公告)号：US06753189B1

公开(公告)日：2004-06-22

申请号：US09325214

申请日：1999-06-03

申请人： Kenji Narahara ,  Toshiyuki Uehara

发明人： Kenji Narahara ,  Toshiyuki Uehara

IPC分类号： G01N33543

CPC分类号： G01N33/558 ,  G01N2333/02 ,  G01N2333/59 ,  Y10S436/81 ,  Y10S436/823 ,  Y10S436/824

摘要： A catcher, having an immunological epitope, is fixed to a detection zone of a spreading layer. A marker, capable of easy detection, has an inmmunological epitope. The marker and bispecific antibodies are soluble so that they can move on the spreading layer. The bispecific antibodies include a first bispecific antibody, having specificity for the detectable material in the fluid sample and the marker, and a second bispecific antibody, having specificity for the detectable material in the fluid sample and the catcher. Pore sizes and particle diameters are set so that the reaction product in which the marking elements and the particles are bonded are caught at a catching section. The concentration of the marking elements and the particles are increased to improve detection sensitivity. The result is an inexpensive and simple detection apparatus being highly-sensitive for the immunological detection of a detectable material, without wasting valuable antibodies.

摘要翻译： 具有免疫表位的捕获器固定在扩散层的检测区上。 能够容易检测的标记物具有无效表位。 标记物和双特异性抗体是可溶的，使得它们可以在扩散层上移动。 双特异性抗体包括对流体样品和标记物中的可检测材料具有特异性的第一双特异性抗体，以及对流体样品和捕获器中的可检测材料具有特异性的第二双特异性抗体。 设定孔径和粒径，使得标记元件和颗粒结合的反应产物被捕获在捕获部分。 标记元件和颗粒的浓度增加以提高检测灵敏度。 结果是廉价且简单的检测装置对于可检测材料的免疫学检测高度敏感，而不浪费有价值的抗体。

公开(公告)号：US08402846B2

公开(公告)日：2013-03-26

申请号：US12087679

申请日：2007-01-16

申请人： Tomohiro Mimori ,  Kenji Narahara

发明人： Tomohiro Mimori ,  Kenji Narahara

IPC分类号： G01N33/48 ,  G01N33/487

CPC分类号： G01N1/286 ,  B02C19/08 ,  G01N2001/2866

摘要： There is provided a sample trituration vessel that even when the sample is relatively hard, enables triturating of the sample easily and with high efficiency. The sample trituration vessel includes a tubular body having an opening at one end and having a bottom part at the other end thereof. The tubular body is furnished with a rugged portion there-inside so as to be able to meet another rugged port formed on a trituration rod. The sample trituration vessel itself can exert shearing effect on the sample. The tubular body is formed of flexible material, and upon application of external force from outside of the tubular body, the tubular body is deformed, thereby breaking down the sample held within the tubular body.

摘要翻译： 提供了样品研磨容器，即使样品相对较硬，也能容易地和高效率地研磨样品。 样品研磨容器包括管状体，其在一端具有开口并且在其另一端具有底部。 管状体在内部设置有粗糙部分，以便能够满足形成在研磨棒上的另一个坚固的端口。 样品研磨容器本身可以对样品施加剪切效应。 管状体由柔性材料形成，并且当从管状体的外部施加外力时，管状体变形，从而破坏保持在管状体内的样品。

公开(公告)号：US20110136142A1

公开(公告)日：2011-06-09

申请号：US12961140

申请日：2010-12-06

申请人： Takayoshi OYAMADA ,  Junichi Katada ,  Kenji Narahara ,  Daisuke Ogata ,  Tomonori Takeshige ,  Akihiko Onodera

发明人： Takayoshi OYAMADA ,  Junichi Katada ,  Kenji Narahara ,  Daisuke Ogata ,  Tomonori Takeshige ,  Akihiko Onodera

IPC分类号： G01N33/53

CPC分类号： G01N33/54393 ,  G01N33/558 ,  G01N33/585

摘要： It is an object of the present invention to provide an immunochromatography method capable of measuring a sample in a low concentration range and a high concentration range by setting the measurable range of the sample much wider than the conventional measurable range. The present invention provides an immunochromatography method, which comprises: spreading a test substance, a first labeling substance modified with a first binding substance that binds to the test substance, and a second labeling substance modified with the first binding substance that binds to the test substance, which are in a mixed state, on an insoluble carrier; and capturing the test substance, the first labeling substance, and/or the second labeling substance at a reaction site on the insoluble carrier having a substance having ability to bind to the first binding substance that binds to the test substance, so as to detect the test substance, wherein the first labeling substance is a labeling substance that causes an amplification reaction, and the second labeling substance is a labeling substance that does not substantially influence on the amplification reaction.

摘要翻译： 本发明的目的是提供一种免疫色谱法，其能够通过将样品的可测量范围设定为比现有的可测量范围宽得多的范围来测量低浓度范围和高浓度范围的样品。 本发明提供了一种免疫色谱法，其包括：扩散测试物质，用与测试物质结合的第一结合物质改性的第一标记物质和与第一结合物质相结合的第二标记物质， 在不溶性载体上处于混合状态; 以及在具有结合到与测试物质结合的第一结合物质的能力的物质的不溶性载体上的反应部位捕获测试物质，第一标记物质和/或第二标记物质，以便检测 测试物质，其中所述第一标记物质是引起扩增反应的标记物质，所述第二标记物质是对扩增反应没有实质影响的标记物质。