公开(公告)号：US10473667B2

公开(公告)日：2019-11-12

申请号：US15175434

申请日：2016-06-07

申请人： Tania Vu ,  Thomas Jacob ,  Brian J Druker

发明人： Tania Vu ,  Thomas Jacob ,  Brian J Druker

IPC分类号： G01N33/48 ,  G01N31/00 ,  G01N33/58 ,  G01N33/68 ,  G01N33/574 ,  B82Y15/00

摘要： Embodiments herein provide methods, apparatuses, and systems for detecting, monitoring, measuring, and/or characterizing the activity of phosphoproteins such as tyrosine kinases (TKs) and downstream proteins in TK signal transduction pathways (e.g., TK pathway proteins). In various embodiments, the methods, apparatuses, and systems may use nanoparticles, such as quantum dots (QD), to detect and/or characterize the abnormally overactive TK signaling pathways that underlie tumorgenesis and tumor progression. In various embodiments, the QD-based methods, apparatuses, and systems may have a sufficiently high degree of sensitivity to enable the identification of new TK signaling pathway markers, for example for use in diagnosing, staging, monitoring, and/or prognosing cancers, or in evaluating the efficacy of cancer therapeutics.

公开(公告)号：US10083341B2

公开(公告)日：2018-09-25

申请号：US14946535

申请日：2015-11-19

申请人： Tania Vu ,  Thomas Jacob

发明人： Tania Vu ,  Thomas Jacob

IPC分类号： G06K9/00 ,  G01N33/50 ,  G06T7/00

CPC分类号： G06K9/00147 ,  G01N33/5011 ,  G06K9/0014 ,  G06T7/0014 ,  G06T2207/10064 ,  G06T2207/20076 ,  G06T2207/30072

摘要： Methods and systems for quantifying cellular activity using labeled probes, e.g., quantum dots, are disclosed. In one example approach, a method for quantifying cellular activity in a sample containing intact cells having labeled complexes comprises receiving images of the sample at a plurality of depths and detecting individual intact cells in the images of the sample at the plurality of depths. For each detected cell, discrete labels may be detected and localized in the cell at each depth, a total number of detected and localized labels may be calculated in the cell, and an activity level of the target molecule for the labeled probe in the cell determined.

公开(公告)号：US20160140382A1

公开(公告)日：2016-05-19

申请号：US14946535

申请日：2015-11-19

申请人： Tania Vu ,  Thomas Jacob

发明人： Tania Vu ,  Thomas Jacob

IPC分类号： G06K9/00 ,  G01N33/50 ,  G06T7/00

CPC分类号： G06K9/00147 ,  G01N33/5011 ,  G06K9/0014 ,  G06T7/0014 ,  G06T2207/10064 ,  G06T2207/20076 ,  G06T2207/30072

摘要： Methods and systems for quantifying cellular activity using labeled probes, e.g., quantum dots, are disclosed. In one example approach, a method for quantifying cellular activity in a sample containing intact cells having labeled complexes comprises receiving images of the sample at a plurality of depths and detecting individual intact cells in the images of the sample at the plurality of depths. For each detected cell, discrete labels may be detected and localized in the cell at each depth, a total number of detected and localized labels may be calculated in the cell, and an activity level of the target molecule for the labeled probe in the cell determined.

摘要翻译： 公开了使用标记探针（例如量子点）来定量细胞活性的方法和系统。 在一个示例性方法中，用于定量含有具有标记复合物的完整细胞的样品中的细胞活性的方法包括在多个深度处接收样品的图像，并在多个深度处检测样品的图像中的单个完整小区。 对于每个检测到的细胞，可以在每个深度处检测和定位离散的标记物并定位在细胞中，可以在细胞中计算检测和定位的标签的总数，并且确定细胞中标记的探针的靶分子的活性水平 。

公开(公告)号：US20160377627A1

公开(公告)日：2016-12-29

申请号：US15175434

申请日：2016-06-07

申请人： Tania Vu ,  Thomas Jacob

发明人： Tania Vu ,  Thomas Jacob

IPC分类号： G01N33/58 ,  G01N33/574

CPC分类号： G01N33/588 ,  B82Y15/00 ,  G01N33/57426 ,  G01N33/57484 ,  G01N33/57496 ,  G01N33/587 ,  G01N33/6842 ,  G01N2333/47 ,  G01N2440/14 ,  G01N2500/20 ,  G01N2800/52

摘要： Embodiments herein provide methods, apparatuses, and systems for detecting, monitoring, measuring, and/or characterizing the activity of phosphoproteins such as tyrosine kinases (TKs) and downstream proteins in TK signal transduction pathways (e.g., TK pathway proteins). In various embodiments, the methods, apparatuses, and systems may use nanoparticles, such as quantum dots (QD), to detect and/or characterize the abnormally overactive TK signaling pathways that underlie tumorgenesis and tumor progression. In various embodiments, the QD-based methods, apparatuses, and systems may have a sufficiently high degree of sensitivity to enable the identification of new TK signaling pathway markers, for example for use in diagnosing, staging, monitoring, and/or prognosing cancers, or in evaluating the efficacy of cancer therapeutics.

摘要翻译： 本文的实施方案提供了用于检测，监测，测量和/或表征TK信号转导途径（例如，TK途径蛋白）中的磷酸化蛋白如酪氨酸激酶（TK）和下游蛋白质的活性的方法，装置和系统。 在各种实施方案中，方法，装置和系统可以使用纳米颗粒，例如量子点（QD）来检测和/或表征作为肿瘤发生和肿瘤进展的基础的异常过度活跃的TK信号通路。 在各种实施方案中，基于QD的方法，装置和系统可以具有足够高的灵敏度以使得能够鉴定新的TK信号传导途径标记，例如用于诊断，分期，监测和/或预后癌症， 或评估癌症治疗的功效。

公开(公告)号：US20140212890A1

公开(公告)日：2014-07-31

申请号：US14019829

申请日：2013-09-06

申请人： Thomas Jacob ,  Tania Vu

发明人： Tania Vu ,  Thomas Jacob

IPC分类号： G01N33/68 ,  G01N33/574 ,  G01N33/58

CPC分类号： G01N33/588 ,  B82Y15/00 ,  G01N33/57426 ,  G01N33/57484 ,  G01N33/57496 ,  G01N33/587 ,  G01N33/6842 ,  G01N2333/47 ,  G01N2440/14 ,  G01N2500/20 ,  G01N2800/52

摘要： Embodiments herein provide methods, apparatuses, and systems for detecting, monitoring, measuring, and/or characterizing the activity of phosphoproteins, such as tyrosine kinases (TKs) and downstream proteins in TK signal transduction pathways (e.g., TK pathway proteins). In various embodiments, the methods, apparatuses, and systems may use nanoparticles, such as quantum dots (QD), to detect and/or characterize the abnormally overactive TK signaling pathways that underlie tumorgenesis and tumor progression. In various embodiments, the QD-based methods, apparatuses, and systems may have a sufficiently high degree of sensitivity to enable the identification of new TK signaling pathway markers, for example for use in diagnosing, staging, monitoring, and/or prognosing cancers, or in evaluating the efficacy of cancer therapeutics.

摘要翻译： 本文的实施方案提供了用于检测，监测，测量和/或表征磷酸化蛋白（例如酪氨酸激酶（TK））和TK信号转导途径（例如，TK途径蛋白）中的下游蛋白质的活性的方法，装置和系统。 在各种实施方案中，方法，装置和系统可以使用纳米颗粒，例如量子点（QD）来检测和/或表征作为肿瘤发生和肿瘤进展的基础的异常过度活跃的TK信号通路。 在各种实施方案中，基于QD的方法，装置和系统可以具有足够高的灵敏度以使得能够鉴定新的TK信号传导途径标记，例如用于诊断，分期，监测和/或预后癌症， 或评估癌症治疗的功效。