公开(公告)号：US20130126552A1

公开(公告)日：2013-05-23

申请号：US13736658

申请日：2013-01-08

Applicant: Tecan Trading AG

Inventor： Nikolaus INGENHOVEN ,  Robert LIEBHARD ,  Adi ZUPPIGER ,  Werner HALG

IPC: G01N1/14

CPC classification number: G01N1/14 ,  B01L3/021 ,  B01L2200/143 ,  B01L2200/146 ,  B01L2400/0487 ,  G01N35/1016 ,  G01N2035/1025 ,  Y10T436/11 ,  Y10T436/113332 ,  Y10T436/115831 ,  Y10T436/2575

Abstract: A method of selecting pipetting parameters of a pipetting device for dispensing or pipetting a specific volume of a liquid sample, includes a fluid column of a fluid chamber of the pipetting device set into oscillation at the beginning of the aspiration, the pressure is monitored with the pressure transducer in the fluid chamber, and the pressure changes generated during aspiration into measuring signals are recorded. These measuring signals are processed by a computer or micro-processor and reproduced as a pressure curve, which is characteristic for the fluid column with the aspirated liquid sample, and which is compared with known pressure curves. Based on this comparison, pipetting parameters of the pipetting device are selected for the dispense or for pipetting a specific volume of the liquid sample. Selectable pipetting parameters include a speed of a movement of a pump piston of the pipetting device.

Abstract translation: 选择用于分配或吸移特定体积的液体样品的移液装置的移液参数的方法包括在抽吸开始时设置成振荡的移液装置的流体室的流体柱，压力由 记录在流体室中的压力传感器，并且记录在抽吸期间产生的测量信号中的压力变化。 这些测量信号由计算机或微处理器处理并作为压力曲线再现，该压力曲线是具有吸入液体样品的流体柱的特征，并且与已知的压力曲线进行比较。 基于该比较，选择移液装置的移液参数用于分配或吸移特定体积的液体样品。 可选择的移液参数包括移液装置的泵活塞的移动速度。