公开(公告)号：US09428754B2

公开(公告)日：2016-08-30

申请号：US14416997

申请日：2013-07-22

申请人： Technische Universität Dresden

发明人： Frank Buchholz ,  Madina Karimova

IPC分类号： C12N15/87 ,  C12N15/52 ,  C12N9/12 ,  C12N15/85 ,  C12N15/90 ,  C07H21/04 ,  C12N15/55 ,  C12N15/63 ,  C12N15/66

CPC分类号： C12N15/907 ,  C12N9/12 ,  C12N9/1241 ,  C12N15/52 ,  C12N15/85 ,  C12N15/902 ,  C12N2320/33 ,  C12Y207/07

摘要： The invention relates to the use of a protein with recombinase activity to catalyze a site-specific DNA recombination and a method for producing a site-specific DNA recombination. The invention is applicable alone or in combination with other recombinase systems for genetic manipulation, for example in medical research. The objective of the invention is solved by the use of a protein with recombinase activity to catalyze a site-specific DNA recombination at, preferably at two, recognition sites that are identical or reverse complementary to each other. The invention also includes a method for producing a site-specific DNA recombination comprising the steps of a) providing a cell comprising at least two recognition sites that are identical or reverse complementary to each other; and b) contacting a protein with recombinase activity with the recognition sites, thereby producing the site-specific DNA-recombination.

公开(公告)号：US20150176012A1

公开(公告)日：2015-06-25

申请号：US14416997

申请日：2013-07-22

申请人： Technische Universität Dresden

发明人： Frank Buchholz ,  Madina Karimova

IPC分类号： C12N15/52 ,  C12N9/12

CPC分类号： C12N15/907 ,  C12N9/12 ,  C12N9/1241 ,  C12N15/52 ,  C12N15/85 ,  C12N15/902 ,  C12N2320/33 ,  C12Y207/07

摘要： The invention relates to the use of a protein with recombinase activity to catalyze a site-specific DNA recombination and a method for producing a site-specific DNA recombination. The invention is applicable alone or in combination with other recombinase systems for genetic manipulation, for example in medical research. The objective of the invention is solved by the use of a protein with recombinase activity to catalyze a site-specific DNA recombination at, preferably at two, recognition sites that are identical or reverse complementary to each other. The invention also includes a method for producing a site-specific DNA recombination comprising the steps of a) providing a cell comprising at least two recognition sites that are identical or reverse complementary to each other; and b) contacting a protein with recombinase activity with the recognition sites, thereby producing the site-specific DNA-recombination.

摘要翻译： 本发明涉及具有重组酶活性的蛋白质用于催化位点特异性DNA重组的用途和用于产生位点特异性DNA重组的方法。 本发明可单独应用或与用于遗传操作的其他重组酶系统组合应用，例如在医学研究中。 本发明的目的是通过使用具有重组酶活性的蛋白质来催化位点特异性DNA重组，优选在两个彼此相同或相互互补的识别位点。 本发明还包括用于产生位点特异性DNA重组的方法，包括以下步骤：a）提供包含彼此相同或相互互补的至少两个识别位点的细胞; 和b）使蛋白质与重组酶活性与识别位点接触，从而产生位点特异性DNA重组。

公开(公告)号：US10253332B2

公开(公告)日：2019-04-09

申请号：US15634949

申请日：2017-06-27

申请人： Technische Universität Dresden

发明人： Frank Buchholz ,  Madina Karimova

IPC分类号： C12N15/87 ,  C12N15/52 ,  C12N15/90 ,  C12N9/12 ,  C12N15/85 ,  C07H21/04 ,  C12N15/55 ,  C12N15/63 ,  C12N15/66

摘要： The invention relates to the use of a protein with recombinase activity to catalyze a site-specific DNA recombination and a method for producing a site-specific DNA recombination. The invention is applicable alone or in combination with other recombinase systems for genetic manipulation, for example in medical research. The objective of the invention is solved by the use of a protein with recombinase activity to catalyze a site-specific DNA recombination at, preferably at two, recognition sites that are identical or reverse complementary to each other. The invention also includes a method for producing a site-specific DNA recombination comprising the steps of a) providing a cell comprising at least two recognition sites that are identical or reverse complementary to each other; and b) contacting a protein with recombinase activity with the recognition sites, thereby producing the site-specific DNA-recombination.

公开(公告)号：US09719109B2

公开(公告)日：2017-08-01

申请号：US15216215

申请日：2016-07-21

申请人： Technische Universität Dresden

发明人： Frank Buchholz ,  Madina Karimova

IPC分类号： C12N15/87 ,  C12N15/52 ,  C12N15/90 ,  C12N9/12 ,  C12N15/85 ,  C07H21/04 ,  C12N15/55 ,  C12N15/63 ,  C12N15/66

CPC分类号： C12N15/907 ,  C12N9/12 ,  C12N9/1241 ,  C12N15/52 ,  C12N15/85 ,  C12N15/902 ,  C12N2320/33 ,  C12Y207/07

摘要： The invention relates to the use of a protein with recombinase activity to catalyze a site-specific DNA recombination and a method for producing a site-specific DNA recombination. The invention is applicable alone or in combination with other recombinase systems for genetic manipulation, for example in medical research. The objective of the invention is solved by the use of a protein with recombinase activity to catalyze a site-specific DNA recombination at, preferably at two, recognition sites that are identical or reverse complementary to each other. The invention also includes a method for producing a site-specific DNA recombination comprising the steps of a) providing a cell comprising at least two recognition sites that are identical or reverse complementary to each other; and b) contacting a protein with recombinase activity with the recognition sites, thereby producing the site-specific DNA-recombination.