公开(公告)号：US20190071658A1

公开(公告)日：2019-03-07

申请号：US15767066

申请日：2016-09-05

申请人： Technische Universitaet Muenchen

发明人： Vladimir Zverlov ,  Wolfgang Schwarz ,  Roman Prechtl ,  Benedikt Leis ,  Claudia Held ,  Wolfgang Liebl

IPC分类号： C12N9/42 ,  C12N15/86 ,  C12P19/14

摘要： The invention relates to mutant variants of the β-glucosidase Cgl T from Thermoanaerobacter brockii and nucleic acids for producing the same. Said mutant variants show significantly increased thermostability and enzyme activity. Furthermore, the invention provides vectors, host cells and methods for producing said mutant variants of the β-glucosidase Cgl T. Also provided are artificial cellulosomes comprising the mutant variants of the β-glucosidase Cgl T and methods for the enzymatic hydrolysis of cellulosic biomass comprising said artificial cellulosomes and/or said mutant variants of the β-glucosidase Cgl T.

公开(公告)号：US10907140B2

公开(公告)日：2021-02-02

申请号：US15767066

申请日：2016-09-05

申请人： Technische Universitaet Muenchen

发明人： Vladimir Zverlov ,  Wolfgang Schwarz ,  Roman Prechtl ,  Benedikt Leis ,  Claudia Held ,  Wolfgang Liebl

IPC分类号： C12N9/42 ,  C12N15/86 ,  C12P19/14

摘要： A mutant β-glucosidase polypeptide exhibits enhanced thermostability and has the amino acid sequence: MAKFPRDFVWGTATSSYQIEGAVNEDGRTPSIWDTFSKTX1GKTYKGHT GDVACDHYHRYKEDVEILKEIGVKAYRFSIAWPRIFPEEGKYNPKGMDF YKKLIDELQKRDIX2PAATIYHWDLPQWAYDKGGGWLNRESIKWYVEYA TKLFEELGDAIPLWITHNEPWCSSILSYGIGEHAPGHKNYREALIAAHH ILLSHGEAVKAFREMNIKGSKIGITLNLTPAYPASEKEEDKLAAQYADG FANRWELDPIFKGNYPEDMMELYSKIIGEFDFIKEGDLETISVPIDFLG X3NYYTRSIVKYDEDSMLKAENVPGPGKRTEMGWEISPESLYDLLKRLD REYTKLPMYITENGAAFKDEVTEDGRVHDDERIEYIKEHLKAAAKFIGE GGNLKGYFVWSLMDNFEWAHGYSKRFGIVYVDYX4TQKRILKDSALWYK EVIX5DDGIED, wherein X1 is selected from E, P, T, M, A, S and G; X2 is selected from V, K, R and H; X3 is selected from I, L, M, P, T and A; X4 is selected from T, E, D, N, Q, M and P; and X5 is selected from L, R, K and H.