公开(公告)号：US07482473B2

公开(公告)日：2009-01-27

申请号：US11470820

申请日：2006-09-07

申请人： Tetsuo Nagano ,  Yasuteru Urano ,  Takuji Shoda

发明人： Tetsuo Nagano ,  Yasuteru Urano ,  Takuji Shoda

IPC分类号： C07D405/00

CPC分类号： C07D405/12 ,  C07D405/14

摘要： A compound represented by the following general formula (I) or a salt thereof [R1 represents hydrogen atom, or a C1-12 alkyl group; R2 represents a C1-12 alkyl group having one or more maleimide groups, an aryl group having two or more maleimide groups, a group represented by the formula (A): —(CH2)n—CO—N(R11)(R12) (R11 and R12 represent hydrogen atom, a C1-12 alkyl group, a C1-12 alkyl group having one or more maleimide groups, or an aryl group having one or more maleimide groups, and n represents an integer of 1 to 6), or a group represented by the formula (B): —(CH2)m—NH—CO—R13 (R13 represents a C1-12 alkyl group having one or more maleimide groups, or an aryl group having one or more maleimide groups, and m represents an integer of 1 to 6); R3 and R5 represent hydrogen atom, or a halogen atom; R4 represents hydroxyl group, a C1-12 acyloxy group, a C1-12 acyloxymethyloxy group, or an amino group], which is useful as a novel fluorescent labeling agent having a property of emitting intense fluorescence only after reacting with a thiol group.

摘要翻译： 由以下通式（I）表示的化合物或其盐[R1表示氢原子或C1-12烷基; R2表示具有一个或多个马来酰亚胺基团的C1-12烷基，具有两个或更多个马来酰亚胺基团的芳基，由式（A）表示的基团： - （CH 2）n -CO-N（R 11）（R 12） （R11和R12表示氢原子，C1-12烷基，具有一个或多个马来酰亚胺基的C1-12烷基或具有一个或多个马来酰亚胺基团的芳基，n表示1至6的整数）， 或由式（B）表示的基团： - （CH 2）m -NH-CO-R 13（R 13表示具有一个或多个马来酰亚胺基团的C1-12烷基或具有一个或多个马来酰亚胺基团的芳基， m表示1〜6的整数）; R3和R5代表氢原子或卤原子; R 4表示羟基，C 1-12酰氧基，C 1-12酰氧基甲氧基或氨基]，其仅在与硫醇基反应后具有发射强荧光性质的新型荧光标记剂是有用的。

公开(公告)号：US20090318707A1

公开(公告)日：2009-12-24

申请号：US12066323

申请日：2006-09-13

申请人： Takuya Matsumoto ,  Takuji Shoda ,  Yasuteru Urano ,  Tetsuo Nagano

发明人： Takuya Matsumoto ,  Takuji Shoda ,  Yasuteru Urano ,  Tetsuo Nagano

IPC分类号： C07F5/02

CPC分类号： C07F5/022 ,  C09B23/04 ,  C09B57/10 ,  G01N21/6428 ,  G01N2021/6439

摘要： A compound represented by the following general formula (I): [wherein R1 and R2 independently represent hydrogen atom, or a group represented by the following formula (A): (wherein X1 and X2 represent hydrogen atom, an alkyl group, an alkoxy group, or a halogen atom), provided that R1 and R2 do not simultaneously represent hydrogen atom; R3 and R6 represent an alkyl group; R4 and R7 represent hydrogen atom, an alkyl group, carboxy group, an alkoxycarbonyl group, or sulfo group; and R5 and R8 represents an alkyl group, an aryl group, an alkoxycarbonyl group, a vinyl group, a thienyl group, or a pyrrolyl group], or a salt thereof, which is usable for efficient screening for a chemical substance having applicability as a catalyst of the reaction of Michael addition.

摘要翻译： 由以下通式（I）表示的化合物：[其中R 1和R 2独立地表示氢原子或由下式（A）表示的基团：（其中X 1和X 2表示氢原子，烷基，烷氧基 或卤素原子），条件是R 1和R 2不同时表示氢原子; R3和R6表示烷基; R4和R7表示氢原子，烷基，羧基，烷氧基羰基或磺基; R5和R8表示烷基，芳基，烷氧基羰基，乙烯基，噻吩基或吡咯基]或其盐，其可用于有效筛选具有适用性的化学物质作为 催化剂的迈克尔加成反应。

公开(公告)号：US20090118471A1

公开(公告)日：2009-05-07

申请号：US12337327

申请日：2008-12-17

申请人： Tetsuo NAGANO ,  Yasuteru Urano ,  Takuji Shoda

发明人： Tetsuo NAGANO ,  Yasuteru Urano ,  Takuji Shoda

IPC分类号： C07K1/107 ,  C07D405/14

CPC分类号： C07D405/12 ,  C07D405/14

摘要： A compound represented by the following general formula (I) or a salt thereof [R1 represents hydrogen atom, or a C1-12 alkyl group; R2 represents a C1-12 alkyl group having one or more maleimide groups, an aryl group having two or more maleimide groups, a group represented by the formula (A): —(CH2)n—CO—N(R11)(R12) (R11 and R12 represent hydrogen atom, a C1-12 alkyl group, a C1-12 alkyl group having one or more maleimide groups, or an aryl group having one or more maleimide groups, and n represents an integer of 1 to 6), or a group represented by the formula (B): —(CH2)m—NH—CO—R13 (R13 represents a C1-12 alkyl group having one or more maleimide groups, or an aryl group having one or more maleimide groups, and m represents an integer of 1 to 6); R3 and R5 represent hydrogen atom, or a halogen atom; R4 represents hydroxyl group, a C1-12 acyloxy group, a C1-12 acyloxymethyloxy group, or an amino group], which is useful as a novel fluorescent labeling agent having a property of emitting intense fluorescence only after reacting with a thiol group.

摘要翻译： 由以下通式（I）表示的化合物或其盐[R1表示氢原子或C1-12烷基; R2表示具有一个或多个马来酰亚胺基团的C1-12烷基，具有两个或更多个马来酰亚胺基团的芳基，由式（A）表示的基团： - （CH 2）n -CO-N（R 11）（R 12） （R11和R12表示氢原子，C1-12烷基，具有一个或多个马来酰亚胺基的C1-12烷基或具有一个或多个马来酰亚胺基团的芳基，n表示1至6的整数）， 或由式（B）表示的基团： - （CH 2）m -NH-CO-R 13（R 13表示具有一个或多个马来酰亚胺基团的C1-12烷基或具有一个或多个马来酰亚胺基团的芳基， m表示1〜6的整数）; R3和R5代表氢原子或卤原子; R 4表示羟基，C 1-12酰氧基，C 1-12酰氧基甲氧基或氨基]，其仅在与硫醇基反应后具有发射强荧光性质的新型荧光标记剂是有用的。

公开(公告)号：US20070197778A1

公开(公告)日：2007-08-23

申请号：US11470820

申请日：2006-09-07

申请人： Tetsuo Nagano ,  Yasuteru Urano ,  Takuji Shoda

发明人： Tetsuo Nagano ,  Yasuteru Urano ,  Takuji Shoda

IPC分类号： C07K14/47 ,  C07D405/14

CPC分类号： C07D405/12 ,  C07D405/14

摘要： A compound represented by the following general formula (I) or a salt thereof [R1 represents hydrogen atom, or a C1-12 alkyl group; R2 represents a C1-12 alkyl group having one or more maleimide groups, an aryl group having two or more maleimide groups, a group represented by the formula (A): —(CH2)n—CO—N(R11)(R12) (R11 and R12 represent hydrogen atom, a C1-12 alkyl group, a C1-12 alkyl group having one or more maleimide groups, or an aryl group having one or more maleimide groups, and n represents an integer of 1 to 6), or a group represented by the formula (B): —(CH2)m—NH—CO—R13 (R13 represents a C1-12 alkyl group having one or more maleimide groups, or an aryl group having one or more maleimide groups, and m represents an integer of 1 to 6); R3 and R5 represent hydrogen atom, or a halogen atom; R4 represents hydroxyl group, a C1-12 acyloxy group, a C1-12 acyloxymethyloxy group, or an amino group], which is useful as a novel fluorescent labeling agent having a property of emitting intense fluorescence only after reacting with a thiol group.

摘要翻译： 由下列通式（I）表示的化合物或其盐[R 1]表示氢原子或C 1-12烷基; R 2表示具有一个或多个马来酰亚胺基团的碳原子数为1〜12的烷基，具有两个以上马来酰亚胺基团的芳基，式（A）所示的基团， ：（CH 2）n -CO-N（R 11）（R 12）（R 12） SUP 11和R 12代表氢原子，C 1-12烷基，C 1-12烷基 具有一个或多个马来酰亚胺基团或具有一个或多个马来酰亚胺基团的芳基，n表示1至6的整数）或由式（B）表示的基团： - （CH 2）2 >）-NH-CO-R 13（R 13）表示C 1-12烷基，其具有 一个或多个马来酰亚胺基团或具有一个或多个马来酰亚胺基团的芳基，m表示1至6的整数）; R 3和R 5代表氢原子或卤素原子; R 4表示羟基，C 1-12酰氧基，C 1-12酰氧基甲氧基或氨基]，其中 作为具有仅与硫醇基反应后发出强荧光的性质的新型荧光标记剂是有用的。

公开(公告)号：US07897787B2

公开(公告)日：2011-03-01

申请号：US12066323

申请日：2006-09-13

申请人： Takuya Matsumoto ,  Takuji Shoda ,  Yasuteru Urano ,  Tetsuo Nagano

发明人： Takuya Matsumoto ,  Takuji Shoda ,  Yasuteru Urano ,  Tetsuo Nagano

IPC分类号： C07F5/02

CPC分类号： C07F5/022 ,  C09B23/04 ,  C09B57/10 ,  G01N21/6428 ,  G01N2021/6439

摘要： A compound represented by the following general formula (I): [wherein R1 and R2 independently represent hydrogen atom, or a group represented by the following formula (A): (wherein X1 and X2 represent hydrogen atom, an alkyl group, an alkoxy group, or a halogen atom), provided that R1 and R2 do not simultaneously represent hydrogen atom; R3 and R6 represent an alkyl group; R4 and R7 represent hydrogen atom, an alkyl group, carboxy group, an alkoxycarbonyl group, or sulfo group; and R5 and R8 represents an alkyl group, an aryl group, an alkoxycarbonyl group, a vinyl group, a thienyl group, or a pyrrolyl group], or a salt thereof, which is usable for efficient screening for a chemical substance having applicability as a catalyst of the reaction of Michael addition.

摘要翻译： 由以下通式（I）表示的化合物：[其中R 1和R 2独立地表示氢原子或由下式（A）表示的基团：（其中X 1和X 2表示氢原子，烷基，烷氧基 或卤素原子），条件是R 1和R 2不同时表示氢原子; R3和R6表示烷基; R4和R7表示氢原子，烷基，羧基，烷氧基羰基或磺基; R5和R8表示烷基，芳基，烷氧基羰基，乙烯基，噻吩基或吡咯基]或其盐，其可用于有效筛选具有适用性的化学物质作为 催化剂的迈克尔加成反应。