公开(公告)号：US20210322984A1

公开(公告)日：2021-10-21

申请号：US17259136

申请日：2019-07-11

Applicant: The Penn State Research Foundation

Inventor： Pak Kin WONG ,  Hui LI

IPC: B01L3/00 ,  C12Q1/04 ,  C12Q1/18 ,  C12Q1/689

Abstract: Provided are devices and methods for detecting the presence, absence, and/or characteristics of bacteria. A microfluidic device for characterizing bacteria is provided. The device contains one or more bacteria trapping channels, and one or more pneumatic channels in contact with the bacteria trapping channels, wherein the one or more pneumatic channels are configured to reduce the height of the one or more bacteria trapping channels to thereby trap bacteria in the one or more bacteria trapping channels if said bacteria are present in a liquid biological sample that is introduced into the microfluidic device. Also provides is a method for identifying bacteria that may be used with the microfluidic device. The method includes contacting a sample that contains bacteria with a carbon nanotube and microwave energy such that a probe is introduced into the bacteria. The probe is a double strand polynucleotide probe having a first strand that includes a detectable label, and a second strand having a quencher moiety that quenches a signal from the detectable label when the probe is double stranded. The method includes allowing hybridization between the labeled strand with a polynucleotide in the bacteria if bacteria are present, and if bacteria are present, detecting a signal from the labeled strand that is hybridized to a polynucleotide in the bacteria to thereby identify the presence of, and/or the type of bacteria in the sample, and if the bacteria are not present, determining a lack of a signal from the labeled strand.

公开(公告)号：US20220274103A1

公开(公告)日：2022-09-01

申请号：US17749720

申请日：2022-05-20

Applicant: The Penn State Research Foundation

Inventor： Pak Kin WONG ,  Tak-Sing WONG ,  Jing WANG ,  Hui LI ,  Yi LU ,  Ying WAN

IPC: B01L3/00

Abstract: A biochemical analysis system capable of sample preparation and processing can include at least one inlet channel having a non-fouling, slippery surface to autonomously transport a fluid sample to a chamber by a geometry of the at least one inlet channel. The at least one inlet channel can include a first end, which is open and exposed, and a second end connected to the chamber for mixing and reaction of the fluid sample, and the at least one inlet channel can include a converging or diverging angle.