公开(公告)号：US20240200141A1

公开(公告)日：2024-06-20

申请号：US17915071

申请日：2021-03-06

Applicant: The Regents of the University of California

Inventor： Hsian-Rong Tseng ,  Yazhen Zhu ,  Na Sun ,  Vatche G Agopian

IPC: C12Q1/6886 ,  C12Q1/6804 ,  C12Q1/6844 ,  C12Q1/6883

CPC classification number: C12Q1/6886 ,  C12Q1/6844 ,  C12Q1/6883 ,  C12Q1/6804 ,  C12Q2600/156 ,  C12Q2600/158

Abstract: Methods for selectively capturing an extracellular vesicle (EV) from a sample. including the steps of: functionalizing a capture agent for the EV with a first molecule from a first bioorthogonal functional group such that the capture agent remains attachable to the EV and the first molecule is also able to bond to a second molecule from a second bioorthogonal functional group. the second molecule being complementary to the first molecule: mixing the functionalized capture agent with the sample such that the functionalized capture agent binds to the EV and such that an activated sample is formed: functionalizing a capture surface with the second molecule: and depositing at least a portion of the activated sample on at least a portion of the functionalized capture surface to thereby selectively capture the EV by binding of the second molecule with the first molecule.