公开(公告)号：US20040119010A1

公开(公告)日：2004-06-24

申请号：US10697991

申请日：2003-10-30

Applicant: The Regents of the University of Colorado

Inventor： M. Benjamin Perryman ,  Steve M. Helmke ,  Mark W. Duncan

IPC: H01J049/00

CPC classification number: G01N33/6848 ,  G01N33/6851

Abstract: The present invention provides for methods of quantitating the amounts of proteins or peptides, including those that are closely related isoforms, using matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS). Measurement of protein concentrations in vivo has been extremely difficult and problematic, and protein concentrations have not been shown to correlate well with mRNA levels, the standard used in the past. The present invention overcomes the deficiencies of prior methodologies by taking advantage of MALDI-TOF-MS technology and applying it to proteins and peptides in a way that allows for accurate, quantitative measurement in vivo of protein or peptide concentrations.

Abstract translation: 本发明提供了使用基质辅助激光解吸/离子化飞行时间质谱（MALDI-TOF-MS）定量蛋白质或肽的量的方法，包括具有密切相关同种型的那些。 蛋白质浓度在体内的测量是非常困难和有问题的，并且蛋白质浓度尚未显示与mRNA水平（以往使用的标准）相关性良好。 本发明通过利用MALDI-TOF-MS技术克服现有方法的缺陷，并以允许蛋白质或肽浓度的体内准确，定量测量的方式将其应用于蛋白质和肽。