公开(公告)号：US20210062165A1

公开(公告)日：2021-03-04

申请号：US17003659

申请日：2020-08-26

Applicant: The Trustees of Princeton University

Inventor： Jose L. Avalos ,  Jared E. Toettcher ,  Clifford P. Brangwynne ,  Evan M. Zhao ,  Maxwell Z. Wilson

IPC: C12N9/02 ,  C12N13/00 ,  C12N15/81

Abstract: Provided herein is a system and method of optogenetically inducibly clustering metabolic enzymes for the production of chemicals using cell factories. More particularly, the described inducible protein clustering approach clusters metabolic enzymes by, e.g., a change in illumination conditions (either a switch from dark to light or from light to dark). Performing this clustering leads to an increase in the production of metabolites by the clustered enzymes. In some embodiments, a light-sensitive domain may be replaced with any inducible domain.