公开(公告)号：US20250027134A1

公开(公告)日：2025-01-23

申请号：US18710260

申请日：2022-11-15

Applicant: The Trustees of Princeton University

Inventor： Cameron MYHRVOLD ,  Gaeun KIM ,  Jon ARIZTI SANZ ,  Ryan MCNULTY

IPC: C12Q1/34

Abstract: Described herein is a method of screening for a nuclease with an altered nuclease activity comprising; a) forming a first compartmentalizing reaction comprising a carrier and a variant nuclease template nucleic acid comprising a coding region for a variant nuclease, and amplifying said coding region for said variant nuclease, to obtain variant nuclease encoding nucleic acid; b) forming a second compartmentalizing reaction comprising said variant nuclease encoding nucleic acid, and performing an in vitro transcription and translation reaction to obtain variant nuclease polypeptides; and c) forming a third compartmentalizing reaction comprising said variant nuclease polypeptides, and assaying said variant nuclease polypeptides for the altered nuclease activity of said nuclease.

公开(公告)号：US20250025575A1

公开(公告)日：2025-01-23

申请号：US18712552

申请日：2022-11-23

Applicant: THE TRUSTEES OF PRINCETON UNIVERSITY

Inventor： Carola Maturana ,  Esteban Engel

IPC: A61K48/00 ,  C12N15/86

Abstract: Provided are polynucleotides comprising a transcriptional control element comprising a promoter sequence having at least 80% sequence identity to SEQ ID NO: 1. SEQ ID NO:2. SEQ ID NO:3 or SEQ ID NO:4 and a cell- or tissue-specific enhancer element. Also provided are compositions (e.g., vectors, viruses, host cells, and pharmaceutical compositions) comprising said polynucleotides, methods that are useful for expressing a target gene in a host cell, and methods that are useful for expressing a target gene in a subject in need thereof.

公开(公告)号：US12180400B2

公开(公告)日：2024-12-31

申请号：US17413271

申请日：2019-10-11

Applicant: The Trustees of Princeton University

Inventor： Barry P. Rand ,  Zhengguo Xiao

IPC: C09K11/06 ,  H01G9/20 ,  H10K30/10 ,  H10K85/30 ,  B82Y20/00 ,  B82Y40/00

Abstract: In one aspect, organic-inorganic nanoparticle compositions are described herein comprising engineered surfaces which, in some embodiments, reduce non-radiative recombination mechanisms, thereby providing optoelectronic devices with enhanced efficiencies. In some embodiments, a nanoparticle composition comprises a layer of organic-inorganic perovskite nanocrystals, the organic-inorganic perovskite nanocrystals comprising surfaces associated with growth passivation ligands and trap passivation ligands, wherein the growth passivation ligands are larger than the trap passivation ligands and are of size unable to incorporate into octahedral corner sites of the perovskite crystal structure.

公开(公告)号：US12174020B2

公开(公告)日：2024-12-24

申请号：US17333474

申请日：2021-05-28

Applicant: The Trustees of Princeton University

Inventor： Wonjae Lee ,  Michael Romalis ,  Vito Giovanni Lucivero ,  Mark Limes ,  Elizabeth Foley ,  Tom Kornack

IPC: G01R33/26 ,  G01C17/28

Abstract: According to various embodiments, a method for reducing heading error in a magnetometer that uses Rb-87 atoms is disclosed. The method includes varying a direction and magnitude of a magnetic field at different spin polarization regimes. According to various embodiments, a magnetometer adapted for reduced heading error is disclosed. The magnetometer includes a multipass cell containing Rb-87 vapor, a pump laser operated in a pulse mode that is synchronous with a Larmor frequency, and two orthogonal probe lasers configured to rotate to vary a direction and magnitude of a magnetic field at different spin polarization regimes.

公开(公告)号：US20240391502A1

公开(公告)日：2024-11-28

申请号：US18483479

申请日：2023-10-09

Applicant: Toyota Research Institute, Inc. ,  The Trustees of Princeton University

Inventor： Guy Rosman ,  Justin Lidard ,  Oswin So ,  Yanxia Zhang ,  Paul M. Drews ,  Jonathan DeCastro ,  Xiongyi Cui ,  Yen-Ling Kuo ,  John J. Leonard ,  Avinash Balachandran ,  Naomi Ehrich Leonard

IPC: B60W60/00

Abstract: Systems and methods are provided trajectory prediction that leverages game-theory to improve coverage of multi-modal predictions. Examples of the systems and methods include obtaining training data including first trajectories for a first plurality of agent devices and first map information of a first environment for a past time horizon and applying the training data to a game-theoretic mode-finding algorithm to generate a mode-finding model for each agent device that predicts modes of the first trajectories. A trajectory prediction model can be trained on the predicted modes as a coverage loss term between predicted modes. Future trajectories can be predicted for a second plurality of agent devices based on applying observed data to the trajectory prediction model. A control signal can then be generated to effectuate an autonomous driving command on an agent device of the second plurality of agent devices based on the predicted future trajectories.

公开(公告)号：US20240390882A1

公开(公告)日：2024-11-28

申请号：US18719574

申请日：2022-12-16

Applicant: THE TRUSTEES OF PRINCETON UNIVERSITY

Inventor： Paul J. Chirik ,  Connor S. MacNeil

IPC: B01J31/24 ,  B01J31/22 ,  C07F15/06

Abstract: In one aspect, methods for the facile synthesis of cationic cobalt complexes for asymmetric hydrogenation of alkenes are provided. Synthetic methods described herein, in some embodiments, provide Co(I) precatalysts on a time scale and yield permitting catalytic evaluation of a significant number of enantiopure ligands.

公开(公告)号：US12151978B2

公开(公告)日：2024-11-26

申请号：US17846300

申请日：2022-06-22

Applicant: The Trustees of Princeton University ,  Heavy Metal LLC

Inventor： Adam Bayne Hopkins ,  Salvatore Torquato

IPC: C04B35/111 ,  B22F1/052 ,  B22F1/065 ,  B22F3/11 ,  B22F7/00 ,  B22F7/02 ,  B23K26/342 ,  B23K26/354 ,  B28B1/00 ,  B29C45/00 ,  B29C64/153 ,  B29C64/165 ,  B29C64/371 ,  B33Y10/00 ,  B33Y70/00 ,  B33Y70/10 ,  C03C3/04 ,  C03C12/00 ,  C04B20/00 ,  C04B28/02 ,  C04B35/547 ,  C04B35/56 ,  C04B35/626 ,  C22C14/00 ,  C22C32/00 ,  C22C38/00 ,  C22C38/02 ,  C22C38/44 ,  C22C38/56 ,  B22F10/28 ,  B22F10/34 ,  B29C64/268 ,  B29K105/00 ,  B33Y30/00 ,  B33Y40/00 ,  C22C1/04 ,  C22C1/08 ,  C22C29/08

Abstract: The present invention relates to granular composite density enhancement, and related methods and compositions. The applications where these properties are valuable include but are not limited to: 1) additive manufacturing (“3D printing”) involving metallic, ceramic, cermet, polymer, plastic, or other dry or solvent-suspended powders or gels, 2) concrete materials, 3) solid propellant materials, 4) cermet materials, 5) granular armors, 6) glass-metal and glass-plastic mixtures, and 7) ceramics comprising (or manufactured using) granular composites.

公开(公告)号：US20240352486A1

公开(公告)日：2024-10-24

申请号：US18754912

申请日：2024-06-26

Applicant: The Trustees of Princeton University

Inventor： Cliff BRANGWYNNE ,  Yongdae SHIN ,  Dan BRACHA

IPC: C12N15/90 ,  C07K14/47 ,  C12N9/22

CPC classification number: C12N15/90 ,  C07K14/47 ,  C12N9/22

Abstract: Disclosed is a system and method for the controlled clustering of gene regulatory proteins at the specific target genomic loci. The technology enables formation of liquid-like droplets closely interfaced with genome organization. This technology can be utilized for perturbing endogenous nuclear structures as well as nucleating synthetic membrane-less assemblies. The technology can also be utilized for inducing or detecting mechanical stresses within the genome.

公开(公告)号：US20240344993A1

公开(公告)日：2024-10-17

申请号：US18523814

申请日：2023-11-29

Applicant: The Trustees of Princeton University

Inventor： Stephen Y. CHOU ,  Fei DING

IPC: G01N21/65 ,  B29C33/42 ,  B29C33/56 ,  B29C37/00 ,  B29C59/02 ,  G01N21/64 ,  G03F7/00

CPC classification number: G01N21/658 ,  B29C33/56 ,  B29C37/0003 ,  B29C59/02 ,  B29C59/026 ,  G01N21/648 ,  G01N21/6486 ,  G03F7/0002 ,  B29C33/424 ,  B29C2059/023 ,  B29K2995/0005

Abstract: Some embodiments of the invention provide methods that can create large area complex patterns for nanoimprint molds without or with very litter of the use of the charged beam or photon beam direct-writing of nanostructures. Some embodiments of the invention use (i) Fourier nanoimprint patterning (FNP), (ii) edge-guided nanopatterning (EGN), and (iii) nanostructure self-perfection, and their combinations.

公开(公告)号：US12083230B2

公开(公告)日：2024-09-10

申请号：US17191341

申请日：2021-03-03

Applicant: THE TRUSTEES OF PRINCETON UNIVERSITY

Inventor： Nikolas T Weissmueller ,  Robert K Prud'Homme

IPC: A61K9/51 ,  B82Y5/00 ,  C08L89/00 ,  C09D189/00

CPC classification number: A61K9/5169 ,  B82Y5/00 ,  C08L89/00 ,  C09D189/00

Abstract: In one aspect, methods of preparing composite nanoparticle compositions are described herein. For example, in some embodiments, a method comprises providing a zein solution stream, an organic fluid stream including at least one additive and at least one buffer fluid stream. The zein solution stream, organic fluid stream and buffer fluid stream are delivered to a chamber for mixing at one or more rates sufficient to flash precipitate composite nanoparticles including the additive encapsulated by a shell comprising the zein.