公开(公告)号：US20250027134A1

公开(公告)日：2025-01-23

申请号：US18710260

申请日：2022-11-15

Applicant: The Trustees of Princeton University

Inventor： Cameron MYHRVOLD ,  Gaeun KIM ,  Jon ARIZTI SANZ ,  Ryan MCNULTY

IPC: C12Q1/34

Abstract: Described herein is a method of screening for a nuclease with an altered nuclease activity comprising; a) forming a first compartmentalizing reaction comprising a carrier and a variant nuclease template nucleic acid comprising a coding region for a variant nuclease, and amplifying said coding region for said variant nuclease, to obtain variant nuclease encoding nucleic acid; b) forming a second compartmentalizing reaction comprising said variant nuclease encoding nucleic acid, and performing an in vitro transcription and translation reaction to obtain variant nuclease polypeptides; and c) forming a third compartmentalizing reaction comprising said variant nuclease polypeptides, and assaying said variant nuclease polypeptides for the altered nuclease activity of said nuclease.