公开(公告)号：US20200206364A1

公开(公告)日：2020-07-02

申请号：US16814120

申请日：2020-03-10

申请人： The Trustees of the University of Pennsylvania

发明人： James Eberwine ,  Philip G. Haydon ,  Jai-Yoon Sul ,  Hajime Takano ,  Chia-Wen Kitty Wu ,  Fanyi Zeng

IPC分类号： A61K48/00 ,  C12N15/87 ,  C12N13/00 ,  C12N15/10

摘要： The present invention includes methods for transferring a multigenic phenotype to a cell by transfecting, preferably by phototransfection, and locally transfecting a cell or a cellular process with a laser while the cell is bathed in a fluid medium comprising two or more nucleic acids, thereby introducing the nucleic acid into the interior of the cell. Expression of the nucleic acids results in a multigenic phenotype in the transfected cell.