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公开(公告)号：US11873312B2
公开(公告)日：2024-01-16
申请号：US17333376
申请日：2021-05-28
发明人： James Eberwine , Ivan J. Dmochowski , Sean B. Yeldell , Julianne C. Griepenburg , Teresa L. Rapp , Jennifer M. Singh , Jai-Yoon Sul , Jaehee Lee
IPC分类号： C12Q1/6823 , C12Q1/6825 , C12Q1/6818 , C07F15/00 , C12N15/113 , C07H23/00 , C12N13/00 , B01J35/00
CPC分类号： C07F15/0053 , B01J35/004 , C07H23/00 , C12N13/00 , C12N15/113 , C12Q1/6818 , C12Q1/6823 , C12Q1/6825 , B01J2531/821 , C12N2310/11 , C12N2310/32 , C12N2310/3233 , C12N2310/351 , C12N2310/3517 , C12N2310/531
摘要： Compositions and methods of capturing one or more nucleic acid molecules of a cell or subcellular compartment are described. In certain aspects, the compositions comprise a caged molecule comprising one or more photolinkers and an antisense oligonucleotide, which when uncaged hybridizes to a target nucleic acid molecule.
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公开(公告)号：US10883082B2
公开(公告)日：2021-01-05
申请号：US14230848
申请日：2014-03-31
发明人： James Eberwine , Jai-Yoon Sul , Chia-Wen Wu , Fanyi Zeng , Junhyong Kim
IPC分类号： C12N5/0793 , C12N5/079 , C12N5/077 , C12N15/85 , C12N15/87
摘要： The present invention includes methods for effecting phenotype conversion in a cell by transfecting the cell with phenotype-converting nucleic acid. Expression of the nucleic acids results in a phenotype conversion in the transfected cell. Preferably the phenotype-converting nucleic acid is a transcriptome, and more preferably an mRNA transcriptome.
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公开(公告)号：US20130273537A1
公开(公告)日：2013-10-17
申请号：US13838504
申请日：2013-03-15
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12Q1/6876 , C12Q2600/124
摘要： The invention provides compositions and methods that permit a hybrid nucleic acid molecule to enter a cell and when specifically activated within the cell, the molecule anneals to endogenous cellular RNA and permits the isolation of the RNA.
摘要翻译： 本发明提供允许杂交核酸分子进入细胞的组合物和方法，并且当在细胞内特异性活化时，分子与内源细胞RNA退火并允许RNA的分离。
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公开(公告)号：US20210292352A1
公开(公告)日：2021-09-23
申请号：US17333376
申请日：2021-05-28
发明人： James Eberwine , Ivan J. Dmochowski , Sean B. Yeldell , Julianne C. Griepenburg , Teresa L. Rapp , Jennifer M. Singh , Jai-Yoon Sul , Jaehee Lee
IPC分类号： C07F15/00 , C12N15/113 , C07H23/00 , C12N13/00 , C12Q1/6823 , C12Q1/6825 , B01J35/00 , C12Q1/6818
摘要： Compositions and methods of capturing one or more nucleic acid molecules of a cell or subcellular compartment are described. In certain aspects, the compositions comprise a caged molecule comprising one or more photolinkers and an antisense oligonucleotide, which when uncaged hybridizes to a target nucleic acid molecule.
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公开(公告)号：US20210031186A1
公开(公告)日：2021-02-04
申请号：US16968230
申请日：2019-02-07
发明人： James Eberwine , Jai-Yoon Sul , Paulo Arratia , David Gagnon , Ram Gona
摘要： The present invention provides microfluidic devices capable of generating a flow of fluids within a chamber. The flow of fluids mix within the chamber to form a gradient of concentrations. The chamber is capable of accepting a cell culture and is accessible to retrieve the cell culture. The microfluidic devices are compatible with standard laboratory microscopes for optical analyses of the chamber.
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公开(公告)号：US20200270633A1
公开(公告)日：2020-08-27
申请号：US16871674
申请日：2020-05-11
发明人： James Eberwine , Jai-Yoon Sul , Chia-Wen Wu , Fanyi Zeng , Junhyong Kim
摘要： The present invention includes methods for effecting phenotype conversion in a cell by transfecting the cell with phenotype-converting nucleic acid. Expression of the nucleic acids results in a phenotype conversion in the transfected cell. Preferably the phenotype-converting nucleic acid is a transcriptome, and more preferably an mRNA transcriptome.
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公开(公告)号：US09540680B2
公开(公告)日：2017-01-10
申请号：US13838504
申请日：2013-03-15
CPC分类号： C12Q1/6806 , C12Q1/6876 , C12Q2600/124
摘要： The invention provides compositions and methods that permit a hybrid nucleic acid molecule to enter a cell and when specifically activated within the cell, the molecule anneals to endogenous cellular RNA and permits the isolation of the RNA.
摘要翻译： 本发明提供允许杂交核酸分子进入细胞的组合物和方法，并且当在细胞内特异性活化时，分子与内源细胞RNA退火并允许RNA的分离。
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公开(公告)号：US20160046958A1
公开(公告)日：2016-02-18
申请号：US14880737
申请日：2015-10-12
发明人： James Eberwine , Jai-Yoon Sul , Tae Kyung Kim , Vickas Patel
IPC分类号： C12N15/85 , A61K31/713 , A61K45/06
CPC分类号： C12N15/85 , A61K31/713 , A61K45/06 , A61K48/00 , C12N5/0622 , C12N5/0657 , C12N2501/998 , C12N2506/00 , C12N2506/08 , C12N2506/1307
摘要： The present invention includes methods for effecting phenotype conversion in a cell by transfecting the cell with phenotype-converting nucleic acid. Expression of the nucleic acids results in a phenotype conversion in the transfected cell. Preferably the phenotype-converting nucleic acid is a transcriptome, and more preferably an mRNA transcriptome.
摘要翻译： 本发明包括通过用表型转化核酸转染细胞来实现细胞中表型转化的方法。 核酸的表达导致转染细胞中的表型转化。 表型转换核酸优选是转录组，更优选mRNA转录组。
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公开(公告)号：US11021502B2
公开(公告)日：2021-06-01
申请号：US15501073
申请日：2015-08-04
发明人： James Eberwine , Ivan J. Dmochowski , Sean B. Yeldell , Julianne C. Griepenburg , Teresa L. Rapp , Jennifer M. Singh , Jai-Yoon Sul , Jaehee Lee
IPC分类号： C07F15/00 , C12N15/113 , C12Q1/6823 , C12Q1/6825 , C07H23/00 , C12N13/00 , B01J35/00 , C12Q1/6818
摘要： Compositions and methods of capturing one or more nucleic acid molecules of a cell or subcellular compartment are described. In certain aspects, the compositions comprise a caged molecule comprising one or more photolinkers and an antisense oligonucleotide, which when uncaged hybridizes to a target nucleic acid molecule.
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公开(公告)号：US10093917B2
公开(公告)日：2018-10-09
申请号：US14985826
申请日：2015-12-31
发明人： James Eberwine , Ulo Langel , Emelia Eiriksdottir , Tiina Peritz [nee Pajunen] , Jai-Yoon Sul , Philip G. Haydon , Junhyong Kim
摘要： The present invention includes compositions, methods and kits for the real-time detection of transcription and translation in live cells, tissues and organisms. The present invention further provides method for the rapid sequencing of nucleic acids without using conventional sequencing techniques or reactions.
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