公开(公告)号：US11332721B2

公开(公告)日：2022-05-17

申请号：US16877277

申请日：2020-05-18

申请人： The United States of America, as represented by the Secretary, Department of Health and Human Services

发明人： Peter L. Collins ,  Ursula J. Buchholz ,  Cindy Luongo

IPC分类号： C12N7/00 ,  A61K39/12 ,  C07K14/005 ,  A61K39/155 ,  A61K39/00

摘要： Provided herein are novel recombinant respiratory syncytial viruses (RSV) having an attenuated phenotype that contain mutations in the M2-2 open reading frame that interfere with the expression of the M2-2 protein. The M2-2 mutations may be present in combination with mutations at other loci. Using methods described herein, combinations of mutations are provided to achieve desired levels of attenuation. The recombinant RSV strains described here are suitable for use as live-attenuated RSV vaccines. Also provided are polynucleotide sequences of the described viruses, as well as methods for producing and using the viruses.

公开(公告)号：US10655109B2

公开(公告)日：2020-05-19

申请号：US16061314

申请日：2016-12-12

申请人： The United States of America, as represented by the Secretary, Department of Health and Human Services

发明人： Peter L. Collins ,  Ursula J. Buchholz ,  Cindy Luongo

IPC分类号： C12N7/00 ,  A61K39/12 ,  C07K14/005 ,  A61K39/155 ,  A61K39/00

摘要： Provided herein are novel recombinant respiratory syncytial viruses (RSV) having an attenuated phenotype that contain mutations in the M2-2 open reading frame that interfere with the expression of the M2-2 protein. The M2-2 mutations may be present in combination with mutations at other loci. Using methods described herein, combinations of mutations are provided to achieve desired levels of attenuation. The recombinant RSV strains described here are suitable for use as live-attenuated RSV vaccines. Also provided are polynucleotide sequences of the described viruses, as well as methods for producing and using the viruses.