公开(公告)号：US20100278880A1

公开(公告)日：2010-11-04

申请号：US12810536

申请日：2008-12-30

申请人： Thierry Legon ,  Sabine Pirotton ,  Gael Placier ,  Gilles Kergoat

发明人： Thierry Legon ,  Sabine Pirotton ,  Gael Placier ,  Gilles Kergoat

IPC分类号： A61K9/00 ,  A61K39/35

CPC分类号： A61K39/35 ,  A61K39/36 ,  A61K2039/542 ,  A61K2039/6087

摘要： Method for the production of a purified extract of natural allergens comprising the steps of extracting a natural source of allergens comprising allergenic proteins, purifying of said extract to remove non-protein components denaturating said purified extract, said purified denaturated extract comprising proteins, wherein the most abundant (w/w) proteins, forming together at least 60% (w/w) of all proteins, are at least two proteins, and all proteins represent at least 60% (w/w) of the dry weight of the purified denaturated extract or a method for the production of a purified extract of natural allergens comprising the steps of hydrolysing a denaturated allergen purifying said allergen hydrolysate to remove peptides with a molecular weight above 10,000 Da and below 1,000 Da in order to obtain a purified hydrolysate where 70%, more preferably 80% of the peptides are between 10,000 Da and 1,000 Da, in the form of an starch based pellet.

摘要翻译： 用于生产天然过敏原纯化提取物的方法，包括以下步骤：提取包含过敏原蛋白质的天然过敏原来源，纯化所述提取物以除去使所述纯化提取物变性的非蛋白质组分，所述纯化的变性提取物包含蛋白质，其中最多 含有至少60％（w / w）的所有蛋白质的丰富的（w / w）蛋白质是至少两种蛋白质，所有蛋白质代表纯化变性干重的至少60％（w / w） 提取物或生产天然过敏原纯化提取物的方法，包括以下步骤：将纯化所述变应原水解产物的变性过敏原水解除去分子量高于10,000Da且低于1,000Da的肽，以获得纯化的水解产物，其中70％ 更优选80％的肽在10,000Da和1,000Da之间，以淀粉基丸的形式。

公开(公告)号：US20090324650A1

公开(公告)日：2009-12-31

申请号：US12308859

申请日：2007-06-28

申请人： Thierry Legon ,  Sabine Pirotton ,  Gael Placier ,  Gilles Kergoat

发明人： Thierry Legon ,  Sabine Pirotton ,  Gael Placier ,  Gilles Kergoat

IPC分类号： A61K39/35 ,  C07K1/00 ,  C07K1/14 ,  C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  C07K1/30 ,  C07K14/00 ,  C07K1/20 ,  A61P37/08

CPC分类号： A61K39/35 ,  A61K39/36 ,  A61K2039/542 ,  C07K14/415 ,  C12P21/06

摘要： A method for the production of a purified extract of natural allergens comprising the steps of a) extracting a natural source of allergens comprising allergenic proteins to form an extract, b) purifying of said extract to remove non-protein components to form a purified extract c) denaturating said purified extract to form a purified denaturated extract, said purified denaturated extract comprising proteins, wherein the most abundant (w/w) proteins, forming together at least 60% (w/w) of all proteins, are at least two proteins, and all proteins represent at least 60% (w/w) of the dry weight of the purified denaturated extract and a method for the production of a purified extract of natural allergens comprising the steps of a) hydrolysing a denaturated allergen to form an allergen hydrolysate, b) purifying said allergen hydrolysate to remove peptides with a molecular weight above 10,000 Da and below 1,000 Da in order to obtain a purified hydrolysate where 70%, more preferably 80% of the peptides are between 10,000 Da and 1,000 Da.

摘要翻译： 一种用于生产天然过敏原纯化提取物的方法，包括以下步骤：a）提取包含过敏原蛋白质的天然过敏原来源以形成提取物，b）纯化所述提取物以除去非蛋白质组分以形成纯化的提取物c ）使所述纯化的提取物变性以形成纯化的变性提取物，所述纯化的变性提取物包含蛋白质，其中形成所有蛋白质至少60％（w / w）的最丰富的（w / w）蛋白质是至少两种蛋白质 ，并且所有蛋白质代表纯化的变性提取物的干重的至少60％（w / w），以及用于生产纯化的天然过敏原提取物的方法，包括以下步骤：a）水解变性变应原以形成变应原 水解物，b）纯化所述变应原水解产物以除去分子量高于10,000Da且低于1,000Da的肽，以获得纯化的水解产物，其中70％，更优选80％ 肽在10,000Da和1,000Da之间。