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公开(公告)号：US11667875B1
公开(公告)日：2023-06-06
申请号：US16562262
申请日：2019-09-05
Applicant: ThinkCyte, Inc.
Inventor： Keiji Nakagawa , Yoko Kawamura
Abstract: The present disclosure provides systems, devices, and methods for flow focusing in a microfluidic device. Flow focusing may be used in detection of objects, for example cells, in a stream of fluid passing through a fluidic device. The systems and devices may comprise a flow channel positioned between two sheath channels configured to direct fluid across the flow channel. Flow focusing microfluidic systems and devices disclosed herein may be robust to alignment errors. Systems and devices of the present disclosure may reduce the displacement of flow from the intended locations due to alignment errors. Also disclosed herein are methods for using such microfluidic systems and devices.
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公开(公告)号：US20220317020A1
公开(公告)日：2022-10-06
申请号：US17847478
申请日：2022-06-23
Applicant: ThinkCyte, Inc.
Inventor： Keiji Nakagawa
IPC: G01N15/14
Abstract: A method of evaluating performance of a flow cytometer configured to use a combination of two or more types of calibration particles having different morphologies from each other, includes a first classification step of classifying the calibration particles from each other based on a first optical characteristic by the flow cytometer which is an evaluation target, a second classification step of classifying the calibration particles from each other based on a second optical characteristic which is classifiable at a spatial resolution lower than a spatial resolution at which the first optical characteristic is classified, and the evaluation step of evaluating one or both of particle classification performance and a resolution of the flow cytometer based on a first classification result assessed in the first classification step and a second classification result assessed in the second classification step.
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