公开(公告)号：US20090155869A1

公开(公告)日：2009-06-18

申请号：US11949724

申请日：2007-12-03

申请人： Thomas Buelter ,  Andrew C. Hawkins ,  Kalib Kersh ,  Peter Meinhold ,  Matthew W. Peters ,  Ezhilkani Subbian

发明人： Thomas Buelter ,  Andrew C. Hawkins ,  Kalib Kersh ,  Peter Meinhold ,  Matthew W. Peters ,  Ezhilkani Subbian

IPC分类号： C12P7/16 ,  C12N1/21

CPC分类号： C12N15/52

摘要： A recombinant microorganism expressing at least a heterologous enzyme of an NADH-dependent pathway for conversion of a carbon source to n-butanol, metabolic intermediate and/or a derivative thereof and capable of producing n-butanol, a metabolic intermediate and/or a derivative thereof at a high yield and related methods. The recombinant microorganism engineered to inactivate a native enzyme of one or more pathways that compete with NADH-dependent heterologous pathway, and/or to balance the NADH-dependent heterologous pathway with respect to NADH production and consumption.

摘要翻译： 至少表达NADH依赖性途径的异源酶的重组微生物，用于将碳源转化为正丁醇，代谢中间体和/或其衍生物，并能够生产正丁醇，代谢中间体和/或衍生物 以高产率和相关方法。 重组微生物被工程化以使与NADH依赖性异源途径竞争的一种或多种途径的天然酶失活，和/或平衡NADH依赖性异源途径相对于NADH生产和消耗。