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    ENGINEERED MICROORGANISMS FOR PRODUCING N-BUTANOL AND RELATED METHODS
    1.
    发明申请
    ENGINEERED MICROORGANISMS FOR PRODUCING N-BUTANOL AND RELATED METHODS 审中-公开
    用于生产N-丁醇的工程微生物及相关方法

    公开(公告)号：US20090155869A1

    公开(公告)日：2009-06-18

    申请号：US11949724

    申请日：2007-12-03

    申请人： Thomas Buelter ,  Andrew C. Hawkins ,  Kalib Kersh ,  Peter Meinhold ,  Matthew W. Peters ,  Ezhilkani Subbian

    发明人： Thomas Buelter ,  Andrew C. Hawkins ,  Kalib Kersh ,  Peter Meinhold ,  Matthew W. Peters ,  Ezhilkani Subbian

    IPC分类号： C12P7/16 ,  C12N1/21

    CPC分类号： C12N15/52

    摘要： A recombinant microorganism expressing at least a heterologous enzyme of an NADH-dependent pathway for conversion of a carbon source to n-butanol, metabolic intermediate and/or a derivative thereof and capable of producing n-butanol, a metabolic intermediate and/or a derivative thereof at a high yield and related methods. The recombinant microorganism engineered to inactivate a native enzyme of one or more pathways that compete with NADH-dependent heterologous pathway, and/or to balance the NADH-dependent heterologous pathway with respect to NADH production and consumption.

    摘要翻译： 至少表达NADH依赖性途径的异源酶的重组微生物，用于将碳源转化为正丁醇，代谢中间体和/或其衍生物，并能够生产正丁醇，代谢中间体和/或衍生物 以高产率和相关方法。 重组微生物被工程化以使与NADH依赖性异源途径竞争的一种或多种途径的天然酶失活，和/或平衡NADH依赖性异源途径相对于NADH生产和消耗。

    ENGINEERED MICROORGANISMS FOR PRODUCING ISOPROPANOL
    2.
    发明申请
    ENGINEERED MICROORGANISMS FOR PRODUCING ISOPROPANOL 审中-公开
    用于生产异丙酚的工程微生物

    公开(公告)号：US20080293125A1

    公开(公告)日：2008-11-27

    申请号：US12106173

    申请日：2008-04-18

    申请人： Ezhilkani Subbian ,  Peter Meinhold ,  Thomas Buelter ,  Andrew C. Hawkins

    发明人： Ezhilkani Subbian ,  Peter Meinhold ,  Thomas Buelter ,  Andrew C. Hawkins

    IPC分类号： C07C31/10 ,  C12N1/21 ,  C12N1/15 ,  C12P7/04 ,  C12N1/19

    CPC分类号： C12P7/04

    摘要： In an embodiment, there is disclosed a recombinant microbial host cell having each of the DNA molecules encoding a polypeptide or group of polypeptides that catalyze the conversion: (i) Acetyl-CoA to Acetate and CoA   (conversion 1) (ii) Acetyl-CoA to Acetoacetyl-CoA and CoA   (conversion 2) (iii) Acetoacetyl-CoA and Acetate to Acetoacetate and Acetyl-CoA   (conversion 3.1) (iv) Acetoacetate to Acetone and CO2   (conversion 4) (v) Acetone and NAD(P)H and H+ to Isopropanol and NAD(P)+   (conversion 5) wherein at least one DNA molecule is heterologous to the microbial host cell and wherein the microbial host cell produces isopropanol. In another embodiment, a method is disclosed for the production of isopropanol including providing a recombinant microbial host cell, the host cell of (i) with a fermentable carbon substrate in a fermentation medium under conditions whereby isopropanol is produced, and recovering the isopropanol.

    摘要翻译： 在一个实施方案中，公开了一种重组微生物宿主细胞，其具有编码催化转化的多肽或多肽组的每个DNA分子：<？in-line-formula description =“In-line Formulas”end =“lead” （i）乙酰CoA与乙酸和CoA（转化1）<？在线配方说明=“在线配方”end =“tail”？> <？in-line-formula description =“In- （ii）乙酰CoA至乙酰乙酰辅酶A和CoA（转化2）<？在线公式描述=“内联式”末端=“尾”→> （iii）乙酰乙酰辅酶A和醋酸乙酰乙酸酯和乙酰辅酶A（转化率3.1）<？在线公式描述=“In-line-formula”=“In-line formula”end =“lead” 线式公式“end =”tail“？> <？in-line-formula description =”In-line Formulas“end =”lead“？>（iv）乙酰乙酸酯与丙酮和二氧化碳（转化4） 公式description =“In-line Formulas”end =“tail”？> <？in-line-formula description =“In-line Formulas”end =“lead”？>（v）丙酮和NAD （P）H和H +与异丙醇和NAD（P）+（转化5）<？在线公式描述=“内联式”末端=“尾”→其中至少一个DNA分子与 微生物宿主细胞，其中微生物宿主细胞产生异丙醇。 在另一个实施方案中，公开了用于生产异丙醇的方法，包括提供重组微生物宿主细胞，宿主细胞（i）在发酵培养基中的可发酵碳底物，其中产生异丙醇的条件下，并回收异丙醇。

    ENGINEERED MICROOGRANISMS CAPABLE OF PRODUCING TARGET COMPOUNDS UNDER ANAEROBIC CONDITIONS
    7.
    发明申请
    ENGINEERED MICROOGRANISMS CAPABLE OF PRODUCING TARGET COMPOUNDS UNDER ANAEROBIC CONDITIONS 审中-公开
    在无机条件下生产目标化合物的工程化微生物

    公开(公告)号：US20120190089A1

    公开(公告)日：2012-07-26

    申请号：US13303884

    申请日：2011-11-23

    申请人： Thomas Buelter ,  Peter Meinhold ,  Reid M. Renny Feldman ,  Andrew C. Hawkins ,  Sabine Bastian ,  Frances Arnold ,  Jun Urano

    发明人： Thomas Buelter ,  Peter Meinhold ,  Reid M. Renny Feldman ,  Andrew C. Hawkins ,  Sabine Bastian ,  Frances Arnold ,  Jun Urano

    IPC分类号： C12P7/16 ,  C12N1/19

    CPC分类号： C12P7/16 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0036 ,  Y02E50/10

    摘要： The present invention is generally provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention.

    摘要翻译： 本发明通常提供重组微生物，其包含能够在需氧和厌氧条件下生产C3-C5醇的工程化代谢途径。 本发明还提供已被突变或修饰以提高其NADH依赖性活性或​​将辅因子偏好从NADPH转变为NADH并在修饰的微生物中表达的酮酸酸还原异构酶。 此外，本发明提供以修饰微生物表达的异丁醛脱氢酶。 还提供了在需氧和厌氧条件下通过使合适的底物与本发明的改性微生物接触来生产有益代谢物的方法。