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    Methods and compositions for the expression of a polynucleotide of interest
    5.
    发明申请
    Methods and compositions for the expression of a polynucleotide of interest 审中-公开
    用于表达感兴趣的多核苷酸的方法和组合物

    公开(公告)号：US20070061917A1

    公开(公告)日：2007-03-15

    申请号：US11508045

    申请日：2006-08-22

    申请人： Billy McCutchen ,  Christine Hazel ,  Donglong Liu ,  Albert Lu ,  Wayne Mehre ,  Paul Olson ,  James Wong

    发明人： Billy McCutchen ,  Christine Hazel ,  Donglong Liu ,  Albert Lu ,  Wayne Mehre ,  Paul Olson ,  James Wong

    IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N5/04 ,  C12N15/87

    CPC分类号： C12N15/8274 ,  C12N9/1092 ,  C12N9/88 ,  C12N15/8205 ,  C12N15/8209 ,  C12N15/8216 ,  C12N15/8271 ,  C12N15/8275 ,  C12N15/8278 ,  C12N15/8286 ,  Y02A40/162

    摘要： Methods and compositions for expressing a polynucleotide of interest are provided. Compositions comprise an enhancer domain set forth in SEQ ID NO: 1, 10, 15, or 16 and active variants and fragments thereof. Further provided are DNA constructs comprising at least one transcriptional enhancer sequence comprising the nucleotide sequence set forth in SEQ ID NO:1, 10, 15, or 16 or an active variant or fragment thereof, operably linked to a heterologous promoter. Such chimeric transcription regulatory regions can be operably linked any polynucleotide of interest. Further provided are cells, plants, plant parts, and germplasm comprising the DNA construct. Methods of using the chimeric transcriptional regulatory region are also provided. In specific embodiments, methods of expressing a polynucleotide of interest, including for example, sequences that confer tolerance to herbicides, and methods to select a cell having the DNA construct are provided.

    摘要翻译： 提供了表达感兴趣的多核苷酸的方法和组合物。 组合物包含SEQ ID NO：1，10，15或16所示的增强子结构域及其活性变体和片段。 还提供了包含至少一个转录增强子序列的DNA构建体，所述转录增强子序列包含可操作地连接到异源启动子的SEQ ID NO：1,0,15或16所示的核苷酸序列或其活性变体或片段。 这样的嵌合转录调控区域可以可操作地连接任何感兴趣的多核苷酸。 还提供了包含DNA构建体的细胞，植物，植物部分和种质。 还提供了使用嵌合转录调节区的方法。 在具体实施方案中，提供了表达目标多核苷酸的方法，包括例如赋予除草剂耐受性的序列，以及选择具有DNA构建体的细胞的方法。

    Maize event DP-098140-6 and compositions and methods for the identification and/or detection thereof
    10.
    发明授权
    Maize event DP-098140-6 and compositions and methods for the identification and/or detection thereof 有权
    玉米事件DP-098140-6及其鉴定和/或检测的组合物和方法

    公开(公告)号：US07897846B2

    公开(公告)日：2011-03-01

    申请号：US12156214

    申请日：2008-05-30

    申请人： Timothy K. Chicoine ,  Jeffery W. Derry ,  Christine B. Hazel ,  Donglong Liu ,  Billy Fred McCutchen ,  Wayne J. Mehre ,  Kenneth A. Peeples ,  David W. Saunders

    发明人： Timothy K. Chicoine ,  Jeffery W. Derry ,  Christine B. Hazel ,  Donglong Liu ,  Billy Fred McCutchen ,  Wayne J. Mehre ,  Kenneth A. Peeples ,  David W. Saunders

    IPC分类号： A01H5/00 ,  C12N15/82

    CPC分类号： C12N15/8275 ,  A01H5/10

    摘要： Compositions and methods related to transgenic glyphosate/ALS inhibitor-tolerant maize plants are provided. Specifically, the present invention provides maize plants having a DP-098140-6 event which imparts tolerance to glyphosate and at least one ALS-inhibiting herbicide. The maize plant harboring the DP-098140-6 event at the recited chromosomal location comprises genomic/transgene junctions having at least the polynucleotide sequence of SEQ ID NO:5 and/or 6. The characterization of the genomic insertion site of the DP-098140-6 event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the maize DP-098140-6 events are provided. Methods and compositions for improving yield are further provided.

    摘要翻译： 提供了与转基因草甘膦/ ALS抑制剂耐受性玉米植物相关的组合物和方法。 具体地说，本发明提供具有赋予草甘膦耐受性的DP-098140-6事件的玉米植物和至少一种抑制ALS的除草剂。 携带所述染色体位置的DP-098140-6事件的玉米植物包含至少具有SEQ ID NO：5和/或6的多核苷酸序列的基因组/转基因接头。DP-098140的基因组插入位点的表征 -6事件提供了增强的育种效率，并且能够使用分子标记来跟踪育种种群中的转基因插入物及其后代。 提供了用于识别，检测和使用玉米DP-098140-6事件的各种方法和组合物。 进一步提供了提高产量的方法和组合物。