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    Methods and compositions for the expression of a polynucleotide of interest
    1.
    发明申请
    Methods and compositions for the expression of a polynucleotide of interest 审中-公开
    用于表达感兴趣的多核苷酸的方法和组合物

    公开(公告)号：US20070061917A1

    公开(公告)日：2007-03-15

    申请号：US11508045

    申请日：2006-08-22

    申请人： Billy McCutchen ,  Christine Hazel ,  Donglong Liu ,  Albert Lu ,  Wayne Mehre ,  Paul Olson ,  James Wong

    发明人： Billy McCutchen ,  Christine Hazel ,  Donglong Liu ,  Albert Lu ,  Wayne Mehre ,  Paul Olson ,  James Wong

    IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N5/04 ,  C12N15/87

    CPC分类号： C12N15/8274 ,  C12N9/1092 ,  C12N9/88 ,  C12N15/8205 ,  C12N15/8209 ,  C12N15/8216 ,  C12N15/8271 ,  C12N15/8275 ,  C12N15/8278 ,  C12N15/8286 ,  Y02A40/162

    摘要： Methods and compositions for expressing a polynucleotide of interest are provided. Compositions comprise an enhancer domain set forth in SEQ ID NO: 1, 10, 15, or 16 and active variants and fragments thereof. Further provided are DNA constructs comprising at least one transcriptional enhancer sequence comprising the nucleotide sequence set forth in SEQ ID NO:1, 10, 15, or 16 or an active variant or fragment thereof, operably linked to a heterologous promoter. Such chimeric transcription regulatory regions can be operably linked any polynucleotide of interest. Further provided are cells, plants, plant parts, and germplasm comprising the DNA construct. Methods of using the chimeric transcriptional regulatory region are also provided. In specific embodiments, methods of expressing a polynucleotide of interest, including for example, sequences that confer tolerance to herbicides, and methods to select a cell having the DNA construct are provided.

    摘要翻译： 提供了表达感兴趣的多核苷酸的方法和组合物。 组合物包含SEQ ID NO：1，10，15或16所示的增强子结构域及其活性变体和片段。 还提供了包含至少一个转录增强子序列的DNA构建体，所述转录增强子序列包含可操作地连接到异源启动子的SEQ ID NO：1,0,15或16所示的核苷酸序列或其活性变体或片段。 这样的嵌合转录调控区域可以可操作地连接任何感兴趣的多核苷酸。 还提供了包含DNA构建体的细胞，植物，植物部分和种质。 还提供了使用嵌合转录调节区的方法。 在具体实施方案中，提供了表达目标多核苷酸的方法，包括例如赋予除草剂耐受性的序列，以及选择具有DNA构建体的细胞的方法。

    Genes encoding proteins with pesticidal activity
    7.
    发明申请
    Genes encoding proteins with pesticidal activity 有权
    编码具有杀虫活性的蛋白质的基因

    公开(公告)号：US20050261188A1

    公开(公告)日：2005-11-24

    申请号：US11108389

    申请日：2005-04-18

    申请人： Andre Abad ,  Ronald Flannagan ,  Rafael Herrmann ,  Theodore Kahn ,  Albert Lu ,  Billy McCutchen ,  James Presnail ,  James Wong ,  Cao-Guo Yu

    发明人： Andre Abad ,  Ronald Flannagan ,  Rafael Herrmann ,  Theodore Kahn ,  Albert Lu ,  Billy McCutchen ,  James Presnail ,  James Wong ,  Cao-Guo Yu

    IPC分类号： C07K14/325 ,  C12N15/32 ,  C12N15/82 ,  G06F19/00 ,  A01N63/00

    CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

    摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding δ-endotoxins having pesticidal activity against insect pests. The invention further provides mutagenized nucleic acids that have been modified to encode pesticidal polypeptides such as endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins that may be optimized as well as pesticidal compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.

    摘要翻译： 本发明提供从编码具有杀虫活性的昆虫害虫的δ-内毒素的苏云金芽孢杆菌菌株获得的核酸及其变体和片段。 本发明进一步提供已被修饰以编码杀虫多肽的诱变核酸，例如具有改善的杀虫活性和/或改变的有害生物特异性的内毒素。 本发明的具体实施方案提供编码可能被优化的杀虫蛋白质的分离的核酸，以及包含本发明的核酸的农药组合物，表达盒和转化的微生物和植物。 这些组合物可用于防治害虫，特别是植物害虫的方法。