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公开(公告)号：US09150625B2
公开(公告)日：2015-10-06
申请号：US13467329
申请日：2012-05-09
IPC分类号： C07K14/415 , C12N15/82
CPC分类号： C07K14/415 , C12N15/8221
摘要： Methods and compositions are provided for targeting a polypeptide of interest to a chloroplast. Recombinant polynucleotides comprising a nucleotide sequence encoding a chimeric chloroplast transit peptide (CTP) operably linked to a heterologous polynucleotide of interest are provided. In specific embodiments, the chimeric CTP comprises an N-terminal domain operably linked to a central domain operably linked to a C-terminal domain of a CTP to form a chimeric chloroplast transit peptide having CTP activity. Recombinant polypeptides encoding the same, as well as, cells, plant cells, plants and seeds are further provided which comprise the recombinant polynucleotides. Methods of use of the various sequences are also provided.
摘要翻译： 提供了将目的多肽靶向叶绿体的方法和组合物。 提供了包含编码与目的异源多核苷酸有效连接的嵌合叶绿体转运肽（CTP）的核苷酸序列的重组多核苷酸。 在具体实施方案中，嵌合CTP包含可操作地连接到可操作地连接到CTP的C-末端结构域的中心结构域的N-末端结构域，以形成具有CTP活性的嵌合叶绿体转运肽。 还提供了编码其的重组多肽，以及细胞，植物细胞，植物和种子，其包含重组多核苷酸。 还提供了使用各种序列的方法。
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公开(公告)号：US20120304336A1
公开(公告)日：2012-11-29
申请号：US13467329
申请日：2012-05-09
CPC分类号： C07K14/415 , C12N15/8221
摘要： Methods and compositions are provided for targeting a polypeptide of interest to a chloroplast. Recombinant polynucleotides comprising a nucleotide sequence encoding a chimeric chloroplast transit peptide (CTP) operably linked to a heterologous polynucleotide of interest are provided. In specific embodiments, the chimeric CTP comprises an N-terminal domain operably linked to a central domain operably linked to a C-terminal domain of a CTP to form a chimeric chloroplast transit peptide having CTP activity. Recombinant polypeptides encoding the same, as well as, cells, plant cells, plants and seeds are further provided which comprise the recombinant polynucleotides. Methods of use of the various sequences are also provided.
摘要翻译： 提供了将目的多肽靶向叶绿体的方法和组合物。 提供了包含编码与目的异源多核苷酸有效连接的嵌合叶绿体转运肽（CTP）的核苷酸序列的重组多核苷酸。 在具体实施方案中，嵌合CTP包含可操作地连接到可操作地连接到CTP的C-末端结构域的中心结构域的N-末端结构域，以形成具有CTP活性的嵌合叶绿体转运肽。 还提供了编码其的重组多肽，以及细胞，植物细胞，植物和种子，其包含重组多核苷酸。 还提供了使用各种序列的方法。
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公开(公告)号：US20110054007A1
公开(公告)日：2011-03-03
申请号：US12868994
申请日：2010-08-26
CPC分类号： A01N57/16 , A01N63/00 , A01N65/20 , A01N65/44 , C12N15/113 , C12N15/8218 , C12N15/8286 , C12N2310/11 , C12N2310/531 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Coleopteran plant pest or a Diabrotica plant pest, decrease the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides set forth in any one of SEQ ID NOS: 1-236 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is D. virgifera virgifera, D. barberi, D. speciosa, or D. undecimpunctata howardi. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
摘要翻译： 提供了使用沉积元件的方法和组合物，当由有害生物摄取的诸如鞘翅目植物病虫害（Diaprotica plant pest）的害虫时，会降低害虫中靶序列的表达。 在具体实施方案中，靶序列的表达降低控制有害生物，因此方法和组合物能够限制对植物的损害。 本发明提供了SEQ ID NO：1-236中任一项所述的各种靶多核苷酸或其活性变体及其片段，其中目标害虫中一个或多个序列的表达降低控制害虫（即具有杀虫活性 活动）。 进一步提供的是沉积元件，当受害虫摄入时，降低靶多肽的水平，从而控制害虫。 在具体实施方案中，害虫是D. virgifera virgifera，D.barberi，D. speciosa或D. undecimpunctata howardi。 还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其它宿主细胞。
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公开(公告)号：US07411112B2
公开(公告)日：2008-08-12
申请号：US10961629
申请日：2004-10-08
申请人： Scott Diehn , Albert Laurence Lu , Steven W. Ritchie , Lynne Sims , Kim R. Ward , Philip Benfey , Jee W. Jung
发明人： Scott Diehn , Albert Laurence Lu , Steven W. Ritchie , Lynne Sims , Kim R. Ward , Philip Benfey , Jee W. Jung
CPC分类号： C12N15/8227
摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a root-preferred promoter for the gene encoding CRWAQ81. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
摘要翻译： 本发明提供调节植物中异源核苷酸序列表达的组合物和方法。 组合物包括用于编码CRWAQ81的基因的根优选启动子的新型核苷酸序列。 提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括将可操作地连接到本发明的根优选启动子的核苷酸序列稳定地并入植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。
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