公开(公告)号：US5672514A

公开(公告)日：1997-09-30

申请号：US588854

申请日：1996-01-19

申请人： Tohru Tsuchiya ,  Taizo Akimoto ,  Keiji Mori ,  Yasushi Kojima ,  Gunter Dietzel ,  Gerhard Petz ,  Andreas Kopke

发明人： Tohru Tsuchiya ,  Taizo Akimoto ,  Keiji Mori ,  Yasushi Kojima ,  Gunter Dietzel ,  Gerhard Petz ,  Andreas Kopke

IPC分类号： G01N33/58 ,  G01N33/48 ,  G01N21/76 ,  G01N23/04

CPC分类号： G01N33/581 ,  G01N33/582 ,  Y10T436/13 ,  Y10T436/143333

摘要： A chemiluminescent detecting method includes the steps of uniformly irradiating with radiation a stimulable phosphor sheet formed with a stimulable phosphor layer containing a stimulable phosphor which can store an energy of radiation and be stimulated by visible light to emit the energy of radiation in a form of light, thereby storing energy of radiation uniformly therein, selectively labeling a biopolymer with a labeling substance which can produce chemiluminescent light by contact of itself and a chemiluminescent substance, causing the biopolymer labeled with the labeling substance and the chemiluminescent substance to come into contact with each other, and exposing the stimulable phosphor sheet to chemiluminescent light produced by the contact of the biopolymer labeled with the labeling substance and the chemiluminescent substance. According to this chemiluminescent detecting method, it is possible to effectively produce information relating to a biopolymer such as information relating to a gene with high accuracy by using a stimulable phosphor sheet which can be easily handled and used for both the chemiluminescent detecting method and the autoradiographic detecting method.

摘要翻译： 化学发光检测方法包括以下步骤：用辐射均匀地照射形成有可刺激的磷光体层的可刺激的磷光体片，所述可刺激的磷光体层含有可刺激的磷光体，其可以存储辐射的能量并且被可见光刺激以以光的形式发射辐射能量 ，从而将辐射能量均匀地储存在其中，用能够通过其本身和化学发光物质接触产生化学发光的标记物质选择性地标记生物聚合物，使标记物质和化学发光物质标记的生物聚合物彼此接触 并将可刺激的荧光体片暴露于由标记物质和化学发光物质标记的生物聚合物的接触产生的化学发光光。 根据该化学发光检测方法，通过使用可以容易地处理并用于化学发光检测方法和放射自显影的可刺激的荧光体片，可以有效地产生与生物聚合物有关的信息，例如与基因相关的信息 检测方法。