公开(公告)号：US20230374479A1

公开(公告)日：2023-11-23

申请号：US18030704

申请日：2021-10-07

Applicant: Tokushima University

Inventor： Keishi OSAKABE ,  Yuriko Osakabe ,  Naoki Wada

IPC: C12N9/22 ,  C12N15/63 ,  C12N15/10

CPC classification number: C12N9/22 ,  C12N15/63 ,  C12N15/102 ,  C12N2310/20 ,  C12N2750/14143

Abstract: Provided are a method for targeting a target nucleotide sequence, etc. said method comprising introducing into a cell: (i) CRISPR type I-D Cas proteins Cas5d, Cas6d and Cas7d and a polypeptide containing the N-terminal HD domain of Cas10d, or nucleic acids encoding the proteins and the polypeptide; (ii) a polypeptide that contains not the N-terminal HD domain of Cas10d but the C-terminal partial sequence of Cas10d, or a nucleic acid encoding the polypeptide; and (iii) a crRNA that contains a sequence capable of forming a base pair with the target nucleotide sequence, or a DNA encoding the crRNA.