公开(公告)号：US4515943A

公开(公告)日：1985-05-07

申请号：US486935

申请日：1983-04-19

申请人： Toyofumi Miya ,  Yukiharu Kobayashi ,  Masao Yano

发明人： Toyofumi Miya ,  Yukiharu Kobayashi ,  Masao Yano

IPC分类号： C07H19/207 ,  C07H21/02 ,  C07H19/20

CPC分类号： C07H19/207

摘要： Novel highly pure and stable crystals of .beta.-nicotinamide-adenine-dinucleotide tetrahydrate (NAD) which is triclinic system and has a space group of P1 or P1 and lattice constants: a=8.861 .ANG., b=11.181 .ANG., c=8.630 .ANG., .alpha.=90.82.degree., .beta.=103.40.degree. and .gamma.=109.71.degree.. The crystalline NAD is prepared by cooling a 20 to 60 w/v % aqueous solution of amorphous NAD, which has preferably been treated with a porous weakly basic anion exchange resin to remove impurities, at a temperature of 0.degree. to 20.degree. C. When the crystalline NAD is added to the aqueous solution as seeds, the desired high pure crystalline NAD is prepared without conducting the treatment of amorphous NAD with the porous weakly basic anion exchange resin. A high pure amorphous NAD is obtained from the crystalline NAD by dissolving the crystalline NAD in water and subjecting the aqueous solution to freeze drying or precipitation with a solvent.

摘要翻译： 具有三斜晶系的β-烟酰胺 - 腺嘌呤二核苷酸四水合物（NAD）的新型高度纯度和稳定的晶体，具有P＆upbar＆1或P1和晶格常数的空间群：a = 8.861 ANGSTROM，b = 11.181 ANGSTROM，c = 8.630 ANGSTROM，α= 90.82°，β= 103.40°，γ= 109.71°。 通过冷却20至60w / v％的无定形NAD的水溶液制备结晶NAD，其优选在多孔弱碱性阴离子交换树脂处理以除去杂质，在0℃至20℃的温度下。当 将结晶NAD作为种子加入到水溶液中，制备所需的高纯度结晶NAD，而不用多孔弱碱性阴离子交换树脂处理无定形NAD。 通过将结晶NAD溶解在水中并使该溶液冷冻干燥或用溶剂沉淀，从结晶NAD获得高纯度无定形NAD。