公开(公告)号：US20100240082A1

公开(公告)日：2010-09-23

申请号：US12726424

申请日：2010-03-18

申请人： Tricia Moser ,  Shib Sankar Basu ,  Rogerio T.N. Prata ,  Hangsik Moon ,  Xuejun Zhong ,  Myoung Kim

发明人： Tricia Moser ,  Shib Sankar Basu ,  Rogerio T.N. Prata ,  Hangsik Moon ,  Xuejun Zhong ,  Myoung Kim

IPC分类号： C12Q1/40 ,  C12Q1/34

CPC分类号： C12Q1/34 ,  C12Q1/40 ,  G01N2333/924 ,  G01N2333/928 ,  G01N2333/942 ,  G01N2333/944

摘要： Methods are disclosed for detecting and measuring polysaccharide-hydrolyzing enzyme activity or concentration by partial hydrolysis using a pre-determined, yet short, incubation time and a pre-determined temperature. The resulting reaction mixture has unique chemical (i.e., reaction products) and physical (i.e., viscosity) properties that can be used to detect or measure the polysaccharide-hydrolyzing enzyme activity or concentration.

摘要翻译： 公开了通过使用预定的但短的孵育时间和预定温度通过部分水解来检测和测量多糖水解酶活性或浓度的方法。 所得反应混合物具有独特的化学（即反应产物）和物理（即粘度）性质，可用于检测或测量多糖水解酶活性或浓度。

公开(公告)号：US20120309038A1

公开(公告)日：2012-12-06

申请号：US13577717

申请日：2011-02-22

申请人： Tricia Moser ,  Rene Quadt

发明人： Tricia Moser ,  Rene Quadt

IPC分类号： G01N11/00

CPC分类号： C12Q1/40 ,  C12N9/2422

摘要： Methods are disclosed for distinguishing and identifying plants by measuring partial hydrolysis of polysaccharides on account of polysaccharide-hydrolyzing enzyme activity at pre-determined incubation times and temperatures. Methods also are disclosed for identifying the source organism of a heterologous polysaccharide-hydrolyzing enzyme in a plant by measuring partial hydrolysis of polysaccharides on account of polysaccharide-hydrolyzing enzyme activity at pre-determined incubation times and temperatures. The reaction mixture has unique chemical and physical properties that can be used to construct viscosity curves for measuring polysaccharide-hydrolyzing enzyme activity. The viscosity curves can be compared among plants to distinguish or identify the plants from one another. Likewise, viscosity curves can be compared among source organisms to identify the source organism of the heterologous polysaccharide-hydrolyzing enzyme in the plant.

摘要翻译： 公开了通过在预定的孵育时间和温度下通过测定多糖水解酶活性的多糖的部分水解来区分和鉴定植物的方法。 公开了通过在预定的孵育时间和温度下通过多糖水解酶活性测定多糖的部分水解来鉴定植物中异源多糖水解酶的源生物体的方法。 反应混合物具有独特的化学和物理性能，可用于构建测定多糖水解酶活性的粘度曲线。 可以在植物之间比较粘度曲线以区分或识别植物。 同样，可以在源生物体之间比较粘度曲线，以鉴定植物中异源多糖水解酶的来源生物体。