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    METHODS FOR DISTINGUISHING AND IDENTIFYING PLANT VARIETIES
    1.
    发明申请
    METHODS FOR DISTINGUISHING AND IDENTIFYING PLANT VARIETIES 审中-公开
    鉴定和鉴定植物品种的方法

    公开(公告)号：US20120309038A1

    公开(公告)日：2012-12-06

    申请号：US13577717

    申请日：2011-02-22

    申请人： Tricia Moser ,  Rene Quadt

    发明人： Tricia Moser ,  Rene Quadt

    IPC分类号： G01N11/00

    CPC分类号： C12Q1/40 ,  C12N9/2422

    摘要： Methods are disclosed for distinguishing and identifying plants by measuring partial hydrolysis of polysaccharides on account of polysaccharide-hydrolyzing enzyme activity at pre-determined incubation times and temperatures. Methods also are disclosed for identifying the source organism of a heterologous polysaccharide-hydrolyzing enzyme in a plant by measuring partial hydrolysis of polysaccharides on account of polysaccharide-hydrolyzing enzyme activity at pre-determined incubation times and temperatures. The reaction mixture has unique chemical and physical properties that can be used to construct viscosity curves for measuring polysaccharide-hydrolyzing enzyme activity. The viscosity curves can be compared among plants to distinguish or identify the plants from one another. Likewise, viscosity curves can be compared among source organisms to identify the source organism of the heterologous polysaccharide-hydrolyzing enzyme in the plant.

    摘要翻译： 公开了通过在预定的孵育时间和温度下通过测定多糖水解酶活性的多糖的部分水解来区分和鉴定植物的方法。 公开了通过在预定的孵育时间和温度下通过多糖水解酶活性测定多糖的部分水解来鉴定植物中异源多糖水解酶的源生物体的方法。 反应混合物具有独特的化学和物理性能，可用于构建测定多糖水解酶活性的粘度曲线。 可以在植物之间比较粘度曲线以区分或识别植物。 同样，可以在源生物体之间比较粘度曲线，以鉴定植物中异源多糖水解酶的来源生物体。

    Identification and characterization of a novel alpha-amylase from maize endosperm
    3.
    发明申请
    Identification and characterization of a novel alpha-amylase from maize endosperm 审中-公开
    来自玉米胚乳的新型α-淀粉酶的鉴定和表征

    公开(公告)号：US20090197323A1

    公开(公告)日：2009-08-06

    申请号：US12317838

    申请日：2008-12-30

    申请人： Martha G. James ,  Alan M. Myers ,  Christophe Colleoni ,  Kevin D. Stokes

    发明人： Martha G. James ,  Alan M. Myers ,  Christophe Colleoni ,  Kevin D. Stokes

    IPC分类号： C08B30/00

    CPC分类号： C12N9/2422 ,  C12N15/8245 ,  C12P19/14

    摘要： SHE, a Starch Hydrolytic Enzyme active in maize endosperm (Zea mays), and the cDNA sequence encoding SHE are disclosed. The specificity of native, purified SHE is similar, in general terms, to previously known alpha-amylases. However, the activity of SHE toward amylopectin results in hydrolysis products that are distinctly different from those of other alpha-amylases. SHE, and its homologous equivalents in other plants such as rice, Arabidopsis, apple and potato, can be used in starch processing for generating different, e.g., larger sized, alpha-limit dextrins for industrial use, as compared to those generated by previously known alpha-amylases or other starch hydrolytic enzymes. In addition, modification of the expression of this enzyme in transgenic maize plants or in other transgenic organisms (including bacteria, yeast, and other plant species) can be useful for the generation of novel starch forms or altered starch metabolism.

    摘要翻译： SHE，在玉米胚乳（Zea mays）中活性的淀粉水解酶，以及编码SHE的cDNA序列。 天然纯化的SHE的特异性通常与以前已知的α-淀粉酶相似。 然而，SHE对支链淀粉的活性导致与其它α-淀粉酶明显不同的水解产物。 SHE及其在其他植物如水稻，拟南芥，苹果和马铃薯中的同源等同物可用于淀粉加工中，用于产生不同的，例如较大尺寸的工业用α限制糊精，与之前已知的 α-淀粉酶或其他淀粉水解酶。 此外，在转基因玉米植物或其他转基因生物（包括细菌，酵母和其他植物物种）中修饰该酶的表达可用于产生新的淀粉形式或改变的淀粉代谢。

    Sugar-regulatory sequences in alpha-amylase genes
    9.
    发明申请
    Sugar-regulatory sequences in alpha-amylase genes 失效
    α-淀粉酶基因中的糖调节序列

    公开(公告)号：US20030033634A1

    公开(公告)日：2003-02-13

    申请号：US10206654

    申请日：2002-07-25

    申请人： The Regents of the University of California

    发明人： Raymond L. Rodriguez

    IPC分类号： A01H005/00 ,  C12N005/04 ,  C12N015/82

    CPC分类号： C12N9/2422 ,  C12N15/8238

    摘要： A chimeric plant promoter which is responsive to sugar depletion or deprivation is disclosed. The promoter includes a promoter element and, carried in the element, a heterologous sequence from the rice Amy3D that is responsive to sugar depletion. Also disclosed is a plant transfecting vector containing the sequence, and plant cell transfected with the vector.

    摘要翻译： 公开了对糖消耗或剥夺有反应的嵌合植物启动子。 启动子包括启动子元件，并且在元件中携带来自对糖消耗有反应的稻米Amy3D的异源序列。 还公开了含有该序列的植物转染载体和用载体转染的植物细胞。